Anti-HA tag抗体[HA-7] (ab49969)
Key features and details
- Mouse monoclonal [HA-7] to HA tag
- Suitable for: ELISA, ICC/IF, IP, WB
- Reacts with: Species independent
- Isotype: IgG1
概述
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产品名称
Anti-HA tag抗体[HA-7]
参阅全部 HA tag 一抗 -
描述
小鼠单克隆抗体[HA-7] to HA tag -
宿主
Mouse -
经测试应用
适用于: ELISA, ICC/IF, IP, WBmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide:
YPYDVPDYA
conjugated to KLH, corresponding to amino acids 98-106 of Influenza A Virus Hemagglutinin HA tag -
阳性对照
- Whole extracts of transfected 293T (human embryonal kidney) cells, expressing HA-tagged fusion protein.
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常规说明
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR275412, GR177296. Lot numbers other than GR275412 and GR177296 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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纯度
Proprietary Purification -
纯化说明
Purified from Tissue culture supernatant”. -
克隆
单克隆 -
克隆编号
HA-7 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab49969于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 1 - 2 µg/ml.
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IP | (1) |
Use a concentration of 0.5 - 2 µg/ml.
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WB | (2) |
Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 63 kDa.
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说明 |
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ELISA
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 - 2 µg/ml. |
IP
Use a concentration of 0.5 - 2 µg/ml. |
WB
Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 63 kDa. |
靶标
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相关性
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
别名
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
图片
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HEK-293 cells overexpressing N-terminal HA tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1 μg/mL Anti-HA tag antibody [HA-7] (ab49969). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
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ab49969 Immunoprecipitating HA tag in HA tagged protein transfected 293T whole cell lysate. 25µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting anti-mouse HRP (ab6789) (1/10000) was used to confirm successful immunoprecipation.
75kD is specific band for HA tagged protein. 120kD, 55kD, 35kD is non specific band for protein A/G bead.
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HEK-293 cells overexpressing C-terminal HA tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1 μg/mL Anti-HA tag antibody [HA-7] (ab49969). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab49969 被引用在 11 文献中.
- Shi YF et al. Interaction between CD9 and PI3K-p85 activates the PI3K/AKT signaling pathway in B-lineage acute lymphoblastic leukemia. Oncol Rep 46:N/A (2021). PubMed: 34036396
- Oury J et al. Mechanism of disease and therapeutic rescue of Dok7 congenital myasthenia. Nature 595:404-408 (2021). PubMed: 34163073
- Harris IS et al. Deubiquitinases Maintain Protein Homeostasis and Survival of Cancer Cells upon Glutathione Depletion. Cell Metab 29:1166-1181.e6 (2019). PubMed: 30799286
- Boassa D et al. Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy. Cell Chem Biol 26:1407-1416.e5 (2019). PubMed: 31378710
- Rangel LT et al. Identification and characterization of putative Aeromonas spp. T3SS effectors. PLoS One 14:e0214035 (2019). PubMed: 31163020