重组Anti-ErbB3 / HER3抗体[E186] (ab32121)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E186] to ErbB3 / HER3
- Suitable for: WB
- Knockout validated
- Reacts with: Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-ErbB3 / HER3抗体[E186]
参阅全部 ErbB3 / HER3 一抗 -
描述
兔单克隆抗体[E186] to ErbB3 / HER3 -
宿主
Rabbit -
特异性
ab32121 recognises ErbB3/HER3. WB samples: should avoid boiling -
经测试应用
适用于: WBmore details
不适用于: ICC/IF,IHC or IP -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide within Human ErbB3/ HER3 aa 1300 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P21860 -
阳性对照
- MCF-7 cell lysate.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E186 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32121于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/500 - 1/1000. Detects a band of approximately 185 kDa (predicted molecular weight: 149 kDa).
|
说明 |
---|
WB
1/500 - 1/1000. Detects a band of approximately 185 kDa (predicted molecular weight: 149 kDa). |
靶标
-
功能
Binds and is activated by neuregulins and NTAK. -
组织特异性
Epithelial tissues and brain. -
疾病相关
Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
发展阶段
Overexpressed in a subset of human mammary tumors. -
结构域
The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins. -
翻译后修饰
Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 2065 Human
- Entrez Gene: 29496 Rat
- Omim: 190151 Human
- SwissProt: P21860 Human
- SwissProt: Q62799 Rat
- Unigene: 118681 Human
- Unigene: 10228 Rat
-
别名
- c erbB 3 antibody
- c erbB3 antibody
- Erb b2 receptor tyrosine kinase 3 antibody
see all
图片
-
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: ERBB3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: MCF7 whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab32121 observed at 185 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab32121 was shown to specifically recognize ERBB3 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when knock out samples were examined. Wild-type and ERBB3 knockout samples were subjected to SDS-PAGE. Ab32121 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
-
Anti-ErbB3 / HER3 antibody [E186] (ab32121) at 1/1000 dilution + MDA-MB-435S (human ductal carcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 149 kDa -
All lanes : Anti-ErbB3 / HER3 antibody [E186] (ab32121) at 2.685 µg/ml (purified)
Lane 1 : MDA-MB-435S (human mammary gland ductal carcinoma melanocyte) whole cell lysate
Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 149 kDa
Exposure time: 37 seconds -
Anti-ErbB3 / HER3 antibody [E186] (ab32121) at 1/500 dilution (unpurified) + MCF-7 cell lysate
Predicted band size: 149 kDa
Observed band size: 185 kDa why is the actual band size different from the predicted?
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (18)
ab32121 被引用在 18 文献中.
- Dan W et al. RIP1-dependent Apoptosis and Differentiation Regulated by Skp2 and Akt/GSK3β in Acute Myeloid Leukemia. Int J Med Sci 19:525-536 (2022). PubMed: 35370472
- Zhang M et al. LncRNA AK020546 protects against cardiac ischemia-reperfusion injury by sponging miR-350-3p. Aging (Albany NY) 13:14219-14233 (2021). PubMed: 33988127
- Bai S et al. Exosomal circ_DLGAP4 promotes diabetic kidney disease progression by sponging miR-143 and targeting ERBB3/NF-?B/MMP-2 axis. Cell Death Dis 11:1008 (2020). PubMed: 33230102
- Zhu J et al. Role of the Nrdp1 in Brain Injury Induced by Chronic Intermittent Hypoxia in Rats via Regulating the Protein Levels of ErbB3. Neurotox Res 38:124-132 (2020). PubMed: 32200526
- Pereira PMR et al. HER2-Targeted PET Imaging and Therapy of Hyaluronan-Masked HER2-Overexpressing Breast Cancer. Mol Pharm 17:327-337 (2020). PubMed: 31804840