Anti-Semaphorin 3A抗体(ab23393)
Key features and details
- Rabbit polyclonal to Semaphorin 3A
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human, Recombinant fragment
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Semaphorin 3A抗体
参阅全部 Semaphorin 3A 一抗 -
描述
兔多克隆抗体to Semaphorin 3A -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Rat, Human, Recombinant fragment -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was affinity purified. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab23393于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (2) |
Use at an assay dependent concentration.
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WB | (3) |
1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 89 kDa).
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说明 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 89 kDa). |
靶标
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功能
Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. -
疾病相关
Hypogonadotropic hypogonadism 16 with or without anosmia -
序列相似性
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain. -
结构域
Strong binding to neuropilin is mediated by the carboxy third of the protein. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 10371 Human
- Entrez Gene: 29751 Rat
- Omim: 603961 Human
- SwissProt: Q14563 Human
- SwissProt: Q63548 Rat
- Unigene: 252451 Human
- Unigene: 44410 Rat
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别名
- Coll 1 antibody
- Coll1 antibody
- Collapsin 1 antibody
see all
图片
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Ab23393 staining human normal ileum. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Lanes 1-2 : Anti-Semaphorin 3A antibody (ab23393) at 1/1000 dilution
Lanes 3-4 : Anti-Semaphorin 3A antibody (ab25999)
Lane 1 : Neonatal rat brain
Lanes 2 & 4 : Human recombinant Sema3A/Fc chimera (95/125 kDa)
Lane 3 : Neonatal rat brain
Predicted band size: 89 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
数据表及文件
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SDS download
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Datasheet download
文献 (58)
ab23393 被引用在 58 文献中.
- Su Y et al. Astrocyte endfoot formation controls the termination of oligodendrocyte precursor cell perivascular migration during development. Neuron 111:190-201.e8 (2023). PubMed: 36384142
- Zheng Y et al. Regulation of Semaphorin3A in the process of cutaneous wound healing. Cell Death Differ 29:1941-1954 (2022). PubMed: 35347234
- Jin T et al. Developmental Hypothyroidism Influences the Development of the Entorhinal-Dentate Gyrus Pathway of Rat Offspring. Endocrinol Metab (Seoul) 37:290-302 (2022). PubMed: 35390249
- Wang T et al. Delivery of miR-654-5p via SonoVue Microbubble Ultrasound Inhibits Proliferation, Migration, and Invasion of Vascular Smooth Muscle Cells and Arterial Thrombosis and Stenosis through Targeting TCF21. Oxid Med Cell Longev 2022:4757081 (2022). PubMed: 35910838
- Obata H et al. Age‑related differences for expression of the nerve‑specific proteins after peripheral nerve injury. Exp Ther Med 24:682 (2022). PubMed: 36185767