Glycolysis Stress Test Companion Assay (ab222945)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Adherent cells
概述
-
产品名称
Glycolysis Stress Test Companion Assay -
检测方法
Fluorescent -
样品类型
Adherent cells -
检测类型
Cell-based (quantitative) -
产品概述
Glycolysis Stress Test Companion Assay (ab222945) allows glycolytic characterization at user-defined glucose concentrations in live cells when used in combination with our Glycolysis Assay [Extracellular Acidification] (ab197244).
The assay uses 2-Deoxyglucose (2-DG) and oligomycin to facilitate an in-depth analysis of cellular glycolytic flux: 2-DG blocks glycolysis through competitive hexokinase inhibition allowing quantification of non-glycolytic extracellular acidification (ECA); oligomycin inhibits ATP synthase, therefore preventing aerobic ATP generation and forcing cells to increase glycolytic flux to meet ATP demand. The levels of such compensatory glycolysis achievable under test conditions can reveal underlying glycolytic perturbation not evident under basal conditions and facilitates assessment of ATP-demand limited glycolysis capability. Glucose oxidase is included in the kit to provide a convenient cell-free ECA assay signal control.
-
说明
Learn more about the full range of assays to measure glycolysis, oxygen consumption, fatty acid oxidation and metabolic flux in live cells.
Or review the full metabolism assay guide for other assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress.
-
平台
Microplate reader
性能
-
存放说明
Please refer to protocols. -
组件 55 tests 2-DG 1 x 600µl Glucose 1 vial Glucose Oxidase Signal Control 1 vial Glucose-free Respiration Buffer 1 tablet Oligomycin 1 vial
相关产品
-
Related Products
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab222945 尚未被引用在任何文献中。