重组Anti-GSDMD抗体[EPR19829] (ab210070)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19829] to GSDMD
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-GSDMD抗体[EPR19829]
参阅全部 GSDMD 一抗 -
描述
兔单克隆抗体[EPR19829] to GSDMD -
宿主
Rabbit -
特异性
The expression of C-terminal of GSDMD is upregulated in response to LPS treatment (PMID: 32109412).
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Recombinant Human GSDMD protein (ab164113), HeLa, 293T, PC-3, and Jurkat whole cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19829 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab210070于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 53 kDa).
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说明 |
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WB
1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 53 kDa). |
靶标
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序列相似性
Belongs to the gasdermin family. - Information by UniProt
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数据库链接
- Entrez Gene: 79792 Human
- Omim: 617042 Human
- SwissProt: P57764 Human
- Unigene: 118983 Human
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别名
- 1810036L03Rik antibody
- DF 5L antibody
- DF5L antibody
see all
图片
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All lanes : Anti-GSDMD antibody [EPR19829] (ab210070) at 1/1000 dilution
Lane 1 : THP-1 (Human monocytic leukemia monocyte) cells were differentiated into macrophage-like cells by incubation in the presence of 50ng/ul PMA for 48h. Then incubated for 90 min without treatment whole cell lysate.
Lane 2 : THP-1 (Human monocytic leukemia monocyte) cells were differentiated into macrophage-like cells by incubation in the presence of 50ng/ul PMA for 48h. Then treated with PrgI and PA (a NAIP-NLRC4 inflammasome trigger) for 90 min whole cell lysate.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 23,53 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer was 5% NFDM/TBST.
The lysates were kindly provided by our collaborator Dr Feng Shao's lab, NIBS.
The 43 kDa band is likely to be a GSDMD cleavage product by caspase-3 at GSDMD Asp88 (PMID: 30902848, PMID: 28392147 , PMID: 30135078).
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All lanes : Anti-GSDMD antibody [EPR19829] (ab210070) at 1/1000 dilution
Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Human Gasdermin-D knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The lysates were kindly provided by our collaborator Dr Feng Shao's lab, NIBS.
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All lanes : Anti-GSDMD antibody [EPR19829] (ab210070) at 1/1000 dilution
Lane 1 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (38)
ab210070 被引用在 38 文献中.
- Shi JH et al. TRK-fused gene (TFG) regulates ULK1 stability via TRAF3-mediated ubiquitination and protects macrophages from LPS-induced pyroptosis. Cell Death Dis 13:93 (2022). PubMed: 35091545
- Li J et al. A 4-Benzene-Indol Derivative Alleviates LPS-Induced Acute Lung Injury Through Inhibiting the NLRP3 Inflammasome. Front Immunol 13:812164 (2022). PubMed: 35222388
- Qiu Y et al. Exogenous spermine inhibits high glucose/oxidized LDL-induced oxidative stress and macrophage pyroptosis by activating the Nrf2 pathway. Exp Ther Med 23:310 (2022). PubMed: 35350102
- Junqueira C et al. FcγR-mediated SARS-CoV-2 infection of monocytes activates inflammation. Nature 606:576-584 (2022). PubMed: 35385861
- Shkarina K et al. Optogenetic activators of apoptosis, necroptosis, and pyroptosis. J Cell Biol 221:N/A (2022). PubMed: 35420640