重组Alexa Fluor® 488荧光Anti-FYB抗体[EP2546Y] (ab206707)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EP2546Y] to FYB
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 488荧光Anti-FYB抗体[EP2546Y]
参阅全部 FYB 一抗 -
描述
Alexa Fluor® 488荧光兔单克隆抗体[EP2546Y] to FYB -
宿主
Rabbit -
偶联物
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human FYB aa 50-150 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O15117 -
阳性对照
- ICC/IF: Jurkat cells
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯度
Affinity purified -
克隆
单克隆 -
克隆编号
EP2546Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab206707于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/100.
This product gave a positive signal in Jurkat cells fixed with 80% methanol (5 min) |
说明 |
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ICC/IF
1/100. This product gave a positive signal in Jurkat cells fixed with 80% methanol (5 min) |
靶标
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功能
Acts as an adapter protein of the FYN and LCP2 signaling cascades in T cells. Modulates the expression of interleukin-2 (IL-2). Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2. -
组织特异性
Expressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. Not expressed in B-cells, nor in non-lymphoid tissues. -
序列相似性
Contains 1 SH3 domain. -
翻译后修饰
T-cell receptor ligation leads to increased tyrosine phosphorylation. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2533 Human
- Omim: 602731 Human
- SwissProt: O15117 Human
- Unigene: 370503 Human
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别名
- ADAP antibody
- Adhesion and degranulation promoting adaptor protein antibody
- FYB 120/130 antibody
see all
图片
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ab206707 staining FYB in Jurkat cells. The cells were fixed with 80% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206707 at 1/100 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab206707 被引用在 1 文献中.
- Pylaeva E et al. During early stages of cancer, neutrophils initiate anti-tumor immune responses in tumor-draining lymph nodes. Cell Rep 40:111171 (2022). PubMed: 35977505