重组Anti-ICAM1抗体[EPR19938] (ab206398)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19938] to ICAM1
- Suitable for: IHC-P, WB
- Reacts with: Rat
Related conjugates and formulations
概述
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产品名称
Anti-ICAM1抗体[EPR19938]
参阅全部 ICAM1 一抗 -
描述
兔单克隆抗体[EPR19938] to ICAM1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Rat spleen, lung, liver and kidney lysates; C6 and L6 whole cell lysates. IHC-P: Rat liver and kidney tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19938 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab206398于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/5000. Detects a band of approximately 80-110 kDa (predicted molecular weight: 60 kDa).
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说明 |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/5000. Detects a band of approximately 80-110 kDa (predicted molecular weight: 60 kDa). |
靶标
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功能
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. -
序列相似性
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
翻译后修饰
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 25464 Rat
- SwissProt: Q00238 Rat
- Unigene: 12 Rat
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别名
- Antigen identified by monoclonal antibody BB2 antibody
- BB 2 antibody
- BB2 antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling ICAM1 with ab206398 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Membranous staining on sinusoidal endothelial cells of rat liver [PMID: 17058232].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling ICAM1 with ab206398 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Positive staining on extracellular matrix and blood vessels of rat kidney [PMID: 9198039] [PMID 7902311].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-ICAM1 antibody [EPR19938] (ab206398) at 1/5000 dilution
Lane 1 : Rat spleen lysate at 20 µg
Lane 2 : Rat lung lysate at 20 µg
Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate at 10 µg
Lane 4 : L6 (Rat skeletal muscle cell line) whole cell lysate at 10 µg
Lane 5 : Rat liver lysate at 20 µg
Lane 6 : Rat kidney lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 60 kDa
Observed band size: 80-110 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-4: 5 seconds; Lanes 5/6: 1 minute.
The observed molecular weight is consistent with the literature (PMID: 1712683).
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (4)
ab206398 被引用在 4 文献中.
- Liu MM et al. Diammonium glycyrrhizinate lipid ligand ameliorates lipopolysaccharide-induced acute lung injury by modulating vascular endothelial barrier function. Exp Ther Med 21:303 (2021). PubMed: 33717246
- Du H et al. Danhong Injection Attenuates Cerebral Ischemia-Reperfusion Injury in Rats Through the Suppression of the Neuroinflammation. Front Pharmacol 12:561237 (2021). PubMed: 33927611
- Xue J et al. The role of dendritic cells regulated by HMGB1/TLR4 signalling pathway in myocardial ischaemia reperfusion injury. J Cell Mol Med 23:2849-2862 (2019). PubMed: 30784177
- Haihua C et al. Cobra Venom Factor-induced complement depletion protects against lung ischemia reperfusion injury through alleviating blood-air barrier damage. Sci Rep 8:10346 (2018). PubMed: 29985461