重组Anti-Salivary alpha amylase抗体[EPR19605] (ab201450)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19605] to Salivary alpha amylase
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Salivary alpha amylase抗体[EPR19605]
参阅全部 Salivary alpha amylase 一抗 -
描述
兔单克隆抗体[EPR19605] to Salivary alpha amylase -
宿主
Rabbit -
特异性
This product reacts with Salivary alpha amylase (P04745), Pancreatic alpha amylase (P04746) and Carcinoid alpha amylase (P19961) due to high sequence homology. -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human salivary alpha-amylase fragment recombinant protein; human pancreatic alpha-amylase fragment recombinant protein; human alpha-carcinoid alpha-amylase fragment recombinant protein; human parotid gland and pancreas lysates. IHC-P: Human parotid gland and pancreas tissues
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19605 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab201450于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 58 kDa).
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说明 |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 58 kDa). |
靶标
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序列相似性
Belongs to the glycosyl hydrolase 13 family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 276 Human
- Entrez Gene: 277 Human
- Entrez Gene: 278 Human
- Omim: 104700 Human
- SwissProt: P04745 Human
- Unigene: 599274 Human
- Unigene: 654437 Human
- Unigene: 655232 Human
see all -
别名
- amylase, salivary, B antibody
- 1 4 alpha D glucan glucanohydrolase antibody
- 1 antibody
see all
图片
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All lanes : Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/10000 dilution
Lane 1 : Human salivary alpha-amylase fragment recombinant protein
Lane 2 : Human pancreatic alpha-amylase fragment recombinant protein
Lane 3 : Human alpha-carcinoid alpha-amylase fragment recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 58 kDa
Observed band size: 22,22,50 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Human salivary alpha-amylase contains aa311-511 with a GST-tag. Human pancreatic alpha-amylase and alpha-carcinoid alpha-amylase fragment recombinant proteins contain aa311-511 with a His-Tag®. These three recombinant proteins were made in-house.
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Lanes 1-3 : Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/20000 dilution
Lanes 4-6 : Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/1000 dilution
Lane 1 : Human parotid gland lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Human fetal heart lysate
Lane 5 : Human fetal kidney lysate
Lane 6 : Human fetal spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 58 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 3261213).
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Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/5000 dilution + Human pancreas lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 58 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded human parotid gland tissue labeling salivary alpha-amylase + pancreatic alpha-amylase + alpha-carcinoid alpha-amylase with ab201450 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human parotid gland is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling salivary alpha-amylase + pancreatic alpha-amylase + alpha-carcinoid alpha-amylase with ab201450 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human pancreas is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human colon tissue with ab201450 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative control: No staining on human colon.
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab201450 被引用在 1 文献中.
- Cheng CC et al. Overexpression of N-acetylglucosaminyltransferase V promotes human parotid gland acinar cell immortalization via the epidermal receptor activation. J Cell Physiol 237:1780-1789 (2022). PubMed: 34806177