重组Alexa Fluor® 488荧光Anti-NEDD8抗体[Y297] (ab201249)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [Y297] to NEDD8
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
概述
-
产品名称
Alexa Fluor® 488荧光Anti-NEDD8抗体[Y297]
参阅全部 NEDD8 一抗 -
描述
Alexa Fluor® 488荧光兔单克隆抗体[Y297] to NEDD8 -
宿主
Rabbit -
偶联物
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- ICC/IF: A673 cells
-
常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Y297 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
- Alexa Fluor® 647 Anti-NEDD8 antibody [Y297] (ab200849)
- Alexa Fluor® 594 Anti-NEDD8 antibody [Y297] (ab207247)
- Alexa Fluor® 405 Anti-NEDD8 antibody [Y297] (ab207248)
- Alexa Fluor® 555 Anti-NEDD8 antibody [Y297] (ab207249)
- Anti-NEDD8 antibody [Y297] - BSA and Azide free (ab220816)
- Anti-NEDD8 antibody [Y297] (ab81264)
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab201249于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/1000.
This product gave a positive signal in A673 cells fixed with 4% formaldehyde (10 min). |
说明 |
---|
ICC/IF
1/1000. This product gave a positive signal in A673 cells fixed with 4% formaldehyde (10 min). |
靶标
-
功能
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. -
组织特异性
Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes. -
序列相似性
Belongs to the ubiquitin family. -
翻译后修饰
Cleavage of precursor form by UCHL3 or SENP8 is necessary for function. -
细胞定位
Nucleus. Mainly nuclear. - Information by UniProt
-
数据库链接
- Entrez Gene: 4738 Human
- Omim: 603171 Human
- SwissProt: Q15843 Human
- Unigene: 531064 Human
-
别名
- FLJ43224 antibody
- MGC104393 antibody
- MGC125896 antibody
see all
图片
-
ab201249 staining NEDD8 in A673 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201249 at a 1/1000 dilution (shown in green) and ab202272, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab201249 尚未被引用在任何文献中。