Alexa Fluor® 647荧光Anti-ATPase Inhibitory Factor 1/IF1抗体[5E2D7] (ab198077)
Key features and details
- Alexa Fluor® 647 Mouse monoclonal [5E2D7] to ATPase Inhibitory Factor 1/IF1
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 647荧光Anti-ATPase Inhibitory Factor 1/IF1抗体[5E2D7]
参阅全部 ATPase Inhibitory Factor 1/IF1 一抗 -
描述
Alexa Fluor® 647荧光小鼠单克隆抗体[5E2D7] to ATPase Inhibitory Factor 1/IF1 -
宿主
Mouse -
偶联物
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Recombinant full length protein corresponding to Rat ATPase Inhibitory Factor 1/IF1.
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阳性对照
- ICC/IF: HepG2 cells.
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常规说明
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Affinity purified -
克隆
单克隆 -
克隆编号
5E2D7 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab198077于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/100.
This product gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min). |
说明 |
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ICC/IF
1/100. This product gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min). |
靶标
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功能
Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. -
序列相似性
Belongs to the ATPase inhibitor family. -
翻译后修饰
Exhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 327699 Cow
- Entrez Gene: 93974 Human
- Entrez Gene: 11983 Mouse
- Entrez Gene: 25392 Rat
- SwissProt: P01096 Cow
- SwissProt: Q9UII2 Human
- SwissProt: O35143 Mouse
- SwissProt: Q03344 Rat
see all -
别名
- ATIF1_HUMAN antibody
- ATP synthase inhibitor protein antibody
- ATPase inhibitor antibody
see all
图片
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ab198077 staining ATPase Inhibitory Factor 1/IF1 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198077 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab198077 尚未被引用在任何文献中。