重组HRP Anti-Synaptophysin抗体[YE269] (ab195520)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [YE269] to Synaptophysin
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Related conjugates and formulations
概述
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产品名称
HRP Anti-Synaptophysin抗体[YE269]
参阅全部 Synaptophysin 一抗 -
描述
HRP兔单克隆抗体[YE269] to Synaptophysin -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab189853) -
阳性对照
- WB: PC12 cell lysate, mouse brain and rat brain lysates. IHC-P: normal human pancreas tissue.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
YE269 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)
- Alexa Fluor® 647 Anti-Synaptophysin antibody [YE269] (ab196166)
- Alexa Fluor® 488 Anti-Synaptophysin antibody [YE269] (ab196379)
- Alexa Fluor® 594 Anti-Synaptophysin antibody [YE269] (ab206868)
- Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] (ab206870)
- Anti-Synaptophysin antibody [YE269] (ab32127)
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab195520于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).Can be blocked with Synaptophysin peptide (ab189853).
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IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).Can be blocked with Synaptophysin peptide (ab189853). |
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. -
组织特异性
Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. -
疾病相关
Mental retardation, X-linked, SYP-related -
序列相似性
Belongs to the synaptophysin/synaptobrevin family.
Contains 1 MARVEL domain. -
结构域
The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein. -
翻译后修饰
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
细胞定位
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome. - Information by UniProt
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数据库链接
- Entrez Gene: 6855 Human
- Entrez Gene: 20977 Mouse
- Entrez Gene: 24804 Rat
- Omim: 313475 Human
- SwissProt: P08247 Human
- SwissProt: Q62277 Mouse
- SwissProt: P07825 Rat
- Unigene: 632804 Human
see all -
别名
- Major synaptic vesicle protein p38 antibody
- MRX96 antibody
- MRXSYP antibody
see all
图片
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All lanes : HRP Anti-Synaptophysin antibody [YE269] (ab195520) at 1/1000 dilution
Lane 1 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 2 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab195520 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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IHC image of Synaptophysin staining in a section of formalin-fixed paraffin-embedded normal human pancreas tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195520 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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