Anti-TNF Receptor I抗体[H398] (ab194814)
Key features and details
- Mouse monoclonal [H398] to TNF Receptor I
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
Anti-TNF Receptor I抗体[H398]
参阅全部 TNF Receptor I 一抗 -
描述
小鼠单克隆抗体[H398] to TNF Receptor I -
宿主
Mouse -
特异性
ab194814 recognizes the extracellular domain of TNF Receptor I. The antibody blocks biological activity of both natural and recombinant Human TNF alpha and TNF beta. -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human TNF Receptor I.
Database link: P19438 -
阳性对照
- Flow Cyt: Human blood.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.095% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
ab194814 was purified from cell culture supernatant by protein-A affinity chromatography. Purity is> 95% (by SDS-PAGE). -
克隆
单克隆 -
克隆编号
H398 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab194814于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use a concentration of 1 - 4 µg/ml.
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说明 |
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Flow Cyt
Use a concentration of 1 - 4 µg/ml. |
靶标
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功能
Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. -
疾病相关
Familial hibernian fever
Multiple sclerosis 5 -
序列相似性
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
结构域
The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP. -
翻译后修饰
The soluble form is produced from the membrane form by proteolytic processing. -
细胞定位
Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 7132 Human
- Omim: 191190 Human
- SwissProt: P19438 Human
- Unigene: 279594 Human
- Unigene: 713833 Human
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别名
- CD120a antibody
- FPF antibody
- MGC19588 antibody
see all
图片
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Flow cytometry surface staining pattern of human peripheral whole blood stained using ab194814 purified antibody at a concentration in sample of 3 µg/mL GAM APC.
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Separation of human monocytes (red-filled) from CD120a negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab194814 purified antibody at concentration in sample of 3 µg/mL GAM APC.
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab194814 被引用在 1 文献中.
- Beliu G et al. Bioorthogonal labeling with tetrazine-dyes for super-resolution microscopy. Commun Biol 2:261 (2019). PubMed: 31341960