重组HRP Anti-CDK1 + CDK2 + CDK3 (phospho T14)抗体[E161] (ab193834)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [E161] to CDK1 + CDK2 + CDK3 (phospho T14)
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
概述
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产品名称
HRP Anti-CDK1 + CDK2 + CDK3 (phospho T14)抗体[E161] -
描述
HRP兔单克隆抗体[E161] to CDK1 + CDK2 + CDK3 (phospho T14) -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human Hodgkin's Lymphoma tissue.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E161 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] - BSA and Azide free (ab219586)
- Alexa Fluor® 488 Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (ab314672)
- Alexa Fluor® 647 Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (ab314673)
- Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (ab32384)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab193834于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/50.
|
说明 |
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IHC-P
1/50. |
靶标
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细胞定位
CDK1: Nucleus. -
数据库链接
- Entrez Gene: 1017 Human
- Entrez Gene: 1018 Human
- Entrez Gene: 983 Human
- Entrez Gene: 12534 Mouse
- Entrez Gene: 12566 Mouse
- Entrez Gene: 362817 Rat
- Entrez Gene: 54237 Rat
- Omim: 116940 Human
see all -
形式
CDK1: CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
图片
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IHC image of CDK1 staining in a section of formalin-fixed paraffin-embedded human Hodgkin's Lymphoma*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab193834 at 1µg/ml. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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