Anti-Kv1.2抗体[K14/16] (ab192758)
Key features and details
- Mouse monoclonal [K14/16] to Kv1.2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
概述
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产品名称
Anti-Kv1.2抗体[K14/16] -
描述
小鼠单克隆抗体[K14/16] to Kv1.2 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal cerebral cortex.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
K14/16 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab192758于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).
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IHC-P |
Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa). |
IHC-P
Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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相关性
Kv1.2 mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which K(+) ions may pass in accordance with their electrochemical gradient. Kv1.2 binds PDZ domains of DLG1, DLG2 and DLG4. The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments. -
细胞定位
Membrane; Multi-pass membrane protein -
数据库链接
- Entrez Gene: 3737 Human
- Entrez Gene: 16490 Mouse
- Entrez Gene: 25468 Rat
- Omim: 176262 Human
- SwissProt: P16389 Human
- SwissProt: P63141 Mouse
- SwissProt: P63142 Rat
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别名
- Akr6a4 antibody
- BK2 antibody
- HBK5 antibody
see all
图片
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IHC image of Kv1.2 staining in Human normal cerebral cortex formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab192758, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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All lanes : Anti-Kv1.2 antibody [K14/16] (ab192758) at 5 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab192758 被引用在 2 文献中.
- Mathieu PA et al. Demyelination-Remyelination in the Central Nervous System: Ligand-Dependent Participation of the Notch Signaling Pathway. Toxicol Sci N/A:N/A (2019). PubMed: 31168611
- Montani L et al. De novo fatty acid synthesis by Schwann cells is essential for peripheral nervous system myelination. J Cell Biol 217:1353-1368 (2018). PubMed: 29434029