重组Anti-FOXP3抗体[EPR15038-69] (ab191416)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15038-69] to FOXP3
- Suitable for: IHC-Fr, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-FOXP3抗体[EPR15038-69]
参阅全部 FOXP3 一抗 -
描述
兔单克隆抗体[EPR15038-69] to FOXP3 -
宿主
Rabbit -
经测试应用
适用于: IHC-Fr, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: FFPE Human normal tissue sections. Human Hodgkin lymphoma tissue. IHC-Fr: Human tonsil tissue sections.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR15038-69 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab191416于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use a concentration of 5 µg/ml.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
Use a concentration of 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
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说明 |
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IHC-Fr
Use a concentration of 5 µg/ml. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa). |
靶标
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功能
Probable transcription factor. Plays a critical role in the control of immune response. -
疾病相关
Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy. -
序列相似性
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 50943 Human
- Omim: 300292 Human
- SwissProt: Q9BZS1 Human
- Unigene: 247700 Human
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别名
- AIID antibody
- DIETER antibody
- Forkhead box P3 antibody
see all
图片
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IHC image of FOXP3 staining in a section of formalin-fixed paraffin-embedded normal human spleen* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab191416, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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All lanes : Anti-FOXP3 antibody [EPR15038-69] (ab191416) at 5 µg/ml
Lane 1 : HEK293T cell lysate
Lane 2 : HEK293T cell lysate overexpressing Human FOXP3
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 47 kDaThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab10563. Antibody binding was detected using Goat anti Rabbit IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
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IHC image of FOXP3 staining in a section of formalin-fixed frozen normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was then incubated with ab191416, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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IHC image of FOXP3 staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab191416, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Immunohistochemical analysis of paraffin-embedded Human Hodgkin lymphoma tissue labeling FOXP3 with ab191416 at 1/250 dilution (0.9 μg/ml) followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Inset: Negative control: using PBS instead of primary antibody.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Tissue Microarrays stained for "Anti-FOXP3 antibody [EPR15038-69]” using "ab191416" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab191416 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (4)
ab191416 被引用在 4 文献中.
- Mizuno T et al. Elevated expression of B7 homolog 4 is associated with disease progression in upper urinary tract urothelial carcinoma. Cancer Immunol Immunother 71:565-578 (2022). PubMed: 34275008
- Zheng J et al. The CXCR4/miR-125b/FoxP3 axis regulates the function of the epithelial barrier via autophagy in allergic rhinitis. Am J Transl Res 12:2570-2584 (2020). PubMed: 32655791
- Zhou S et al. High Expression of Angiopoietin-like Protein 4 in Advanced Colorectal Cancer and its Association with Regulatory T Cells and M2 Macrophages. Pathol Oncol Res N/A:N/A (2019). PubMed: 31264122
- Liu H et al. Oxymatrine-mediated maturation of dendritic cells leads to activation of FOXP3+/CD4+ Treg cells and reversal of cisplatin-resistance in lung cancer cells. Mol Med Rep 19:4081-4090 (2019). PubMed: 30896871