Anti-Lambda Light chain抗体[LcN-2] (ab187370)
Key features and details
- Mouse monoclonal [LcN-2] to Lambda Light chain
- Suitable for: IHC-P, ICC, WB
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-Lambda Light chain抗体[LcN-2]
参阅全部 Lambda Light chain 一抗 -
描述
小鼠单克隆抗体[LcN-2] to Lambda Light chain -
宿主
Mouse -
特异性
ab187370 is specific to lambda light chain of immunoglobulin and shows no cross-reaction with kappa light chain or any of the five heavy chains. -
经测试应用
适用于: IHC-P, ICC, WBmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) corresponding to Human Lambda Light chain. (Purified Human IgG).
Database link: P01701 -
阳性对照
- WB: Human intestine lysate; IHC-P: Human tonsil tissue; ICC: Ramos cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
ab187370 was purified from Bioreactor Concentrate by Protein A/G. -
克隆
单克隆 -
克隆编号
LcN-2 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab187370于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 0.25 - 0.5 µg/ml.
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes. |
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ICC |
Use a concentration of 1 - 2 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 23 kDa.
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说明 |
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IHC-P
Use a concentration of 0.25 - 0.5 µg/ml. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes. |
ICC
Use a concentration of 1 - 2 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 23 kDa. |
靶标
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相关性
All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 3535 Human
- Entrez Gene: 3546 Human
- Entrez Gene: 8217 Human
- Omim: 147220 Human
- SwissProt: P01701 Human
- SwissProt: P01842 Human
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别名
- Bence Jones protein antibody
- BJP antibody
- Constant region of lambda light chains antibody
see all
图片
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Immunocytochemistry analysis of Ramos (human Burkitt's lymphoma cell line) cells using ab187370 (AF488-labeled) (Green). DAPI was used to stain the cell nuclei (blue). Imaged using confocal microscopy.
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Anti-Lambda Light chain antibody [LcN-2] (ab187370) at 1 µg/ml + Human intestine lysate
Predicted band size: 23 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin embedded Human tonsil tissue labeling Lambda Light chain with ab187370 at 1 µg/ml.
数据表及文件
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Datasheet download
文献 (2)
ab187370 被引用在 2 文献中.
- Xu G et al. Serum lipocalin-2 is a potential biomarker for the clinical diagnosis of nonalcoholic steatohepatitis. Clin Mol Hepatol 27:329-345 (2021). PubMed: 33465844
- Zhang YL et al. Folic acid supplementation acts as a chemopreventive factor in tumorigenesis of hepatocellular carcinoma by inducing H3K9Me2-dependent transcriptional repression of LCN2. Oncotarget 12:366-378 (2021). PubMed: 33659047