Anti-RAD18抗体(ab186835)
Key features and details
- Rabbit polyclonal to RAD18
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-RAD18抗体
参阅全部 RAD18 一抗 -
描述
兔多克隆抗体to RAD18 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant full length protein corresponding to Human RAD18 aa 1-495.
Database link: Q9NS91 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab186835于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
1/500 - 1/2000. Predicted molecular weight: 56 kDa.
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说明 |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/500 - 1/2000. Predicted molecular weight: 56 kDa. |
靶标
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功能
E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the RAD18 family.
Contains 1 RING-type zinc finger.
Contains 1 SAP domain.
Contains 1 UBZ-type zinc finger. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 56852 Human
- Entrez Gene: 58186 Mouse
- Omim: 605256 Human
- SwissProt: Q9NS91 Human
- SwissProt: Q9QXK2 Mouse
- Unigene: 375684 Human
- Unigene: 103812 Mouse
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别名
- 2810024C04Rik antibody
- DNA repair protein rad18 antibody
- E3 ubiquitin-protein ligase RAD18 antibody
see all
图片
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Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cell using ab186835. Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
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Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186835. Blue DAPI for nuclear staining.
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All lanes : Anti-RAD18 antibody (ab186835) at 1/500 dilution
Lane 1 : Extracts from K562 cell line
Lane 2 : Extracts from MCF7 cell line
Lane 3 : Extracts from Jurkat cell line
Lane 4 : Extracts from 293T cell line
Lane 5 : Extracts from mouse liver
Predicted band size: 56 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab186835 被引用在 2 文献中.
- Wu B et al. High expression of RAD18 in glioma induces radiotherapy resistance via down-regulating P53 expression. Biomed Pharmacother 112:108555 (2019). PubMed: 30798132
- Lou P et al. RAD18 contributes to the migration and invasion of human cervical cancer cells via the interleukin-1ß pathway. Mol Med Rep 20:3415-3423 (2019). PubMed: 31432163