人IL-10 ELISA试剂盒(Interleukin-10) (ab185986)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.4 pg/ml
- Range: 9.4 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IL-10 ELISA试剂盒(Interleukin-10)
参阅全部 IL-10 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% supernatant 5 5.2% 批次间 样品 n Mean SD CV% supernatant 3 3.2% -
样品类型
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
1.4 pg/ml -
范围
9.4 pg/ml - 3000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 108 105% - 111% Serum 109 108% - 112% Cell culture media 106 102% - 111% Hep Plasma 116 113% - 117% EDTA Plasma 109 103% - 118% Cit plasma 111 105% - 119% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human
不与反应: Cow -
产品概述
Human IL-10 ELISA Kit (Interleukin-10) (ab185986) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-10 (Interleukin-10) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-10 (Interleukin-10) with 1.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Interleukin 10 (IL-10) is a cytokine involved in immunoregulation through the promotion of Th2 response and inhibition of Th1 responses. It is produced by cells of hematopoietic origin, keratinocytes, placental cytotrophoblasts, and hepatic stellate cells.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 1 x 384 tests 1 x 96 tests 10X Human IL-10 Capture Antibody 1 x 600µl 1 x 600µl 1 x 600µl 10X Human IL-10 Detector Antibody 1 x 600µl 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 1 unit 0 x 0 unit 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 6 x 1ml 1 x 1ml Antibody Diluent CPI2 1 x 6ml 1 x 6ml 1 x 6ml Human IL-10 Lyophilized Recombinant Protein 2 vials 2 vials 2 vials Plate Seals 1 unit 1 unit 1 unit Sample Diluent NBP 1 x 20ml 1 x 20ml 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml 1 x 250ml 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 0 x 0 unit 1 unit Stop Solution 1 x 12ml 2 x 12ml 1 x 12ml TMB Development Solution 1 x 12ml 2 x 12ml 1 x 12ml -
研究领域
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功能
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
组织特异性
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
序列相似性
Belongs to the IL-10 family. -
细胞定位
Secreted. - Information by UniProt
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别名
- CSIF
- Cytokine synthesis inhibitory factor
- GVHDS
see all -
数据库链接
- Entrez Gene: 3586 Human
- Omim: 124092 Human
- SwissProt: P22301 Human
- Unigene: 193717 Human
相关产品
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Alternative Versions
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ELISA kits
图片
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Interpolated concentration of native IL-10 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% stimulated PBMC supernatant (1.5% PHA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
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Example of human IL-10 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human IL-10 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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Human IL-10 concentration was interpolated from the IL-10 standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-10 ELISA Kit (Interleukin-10) (ab185986). Wild-type and IL-10 knockout THP-1 (ab273763) vehicle control and PMA (100 ng/mL, 56 h) + LPS (1 µg/mL, 24 h) treated cells were assessed in duplicate (n=2). Data are represented as the mean and error bars represent standard deviation.”
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Standard Curve comparison between human IL-10 current (new) SimpleStep ELISA kit and original ELISA kit. The current SimpleStep ELISA kit shows increased sensitivity.
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The IL-10 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The IL-10 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of IL-10 were measured in duplicates, interpolated from the IL-10 standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated PBMC cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-10 concentration was determined to be 462 pg/ml in stimulated PBMC cell culture supernatant. PBMC cell culture supernatant samples were stimulated for 46 hours with 1.5% PHA. Unstimulated PBMC cell culture supernatant samples were also measured; all values were below the detectable range of the assay.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (19)
ab185986 被引用在 19 文献中.
- Gao LL et al. Emodin Promotes Autophagy and Prevents Apoptosis in Sepsis-Associated Encephalopathy through Activating BDNF/TrkB Signaling. Pathobiology 89:135-145 (2022). PubMed: 34872094
- Niu F et al. Histone demethylase KDM5A regulates the functions of human periodontal ligament stem cells during periodontitis via the miR-495-3p/HOXC8 axis. Regen Ther 20:95-106 (2022). PubMed: 35509266
- Piao H et al. A positive feedback loop between gastric cancer cells and tumor-associated macrophage induces malignancy progression. J Exp Clin Cancer Res 41:174 (2022). PubMed: 35562774
- Han J et al. Mechanism of circHIPK3-miRNA-124-3p/miRNA-148b-3p-Mediated Inflammatory Responses and Cell Senescence in Candida albicans-Induced Septic Acute Kidney Injury. Gerontology 68:1145-1165 (2022). PubMed: 35576907
- Huang Y et al. Molecular mechanism of lncRNA SNHG12 in immune escape of non-small cell lung cancer through the HuR/PD-L1/USP8 axis. Cell Mol Biol Lett 27:43 (2022). PubMed: 35658874