人Resistin ELISA试剂盒(ab183364)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 24 pg/ml
- Range: 78.1 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Resistin ELISA试剂盒
参阅全部 Resistin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 9 3% 批次间 样品 n Mean SD CV% Serum 3 6% -
样品类型
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
24 pg/ml -
范围
78.1 pg/ml - 5000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 94 88% - 100% Cell culture media 106 106% - 107% Hep Plasma 95 84% - 110% EDTA Plasma 96 88% - 104% Cit plasma 78 68% - 90% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human Resistin ELISA kit (ab183364) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Resistin protein in human serum, plasma and cell culture supernatants. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Resistin with 24 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits. -
说明
Resistin, otherwise known as Found in Inflammatory Zone 3 (FIZZ3), and Adipocyte Secreted Factor (ADSF), is a 114 amino-acid cysteine-rich protein. It can be found as a high molecular weight hexamer or as a more active, lower molecular weight complex. Resistin is the best known member of the family of Resistin-like Molecules (RELMs), which include RELM-alpha/FIZZ1 and RELM-beta/FIZZE 2, both described only in rodents. Furthermore, resistin has been categorized as part of the group of adipokines hormones, which are known to be involved in the development of obesity, insulin resistance, diabetes, inflammation, autoimmune disease and metabolic syndrome.
In Humans, Resistin is a pro-inflammatory molecule primarily secreted by macrophages and mononuclear bone marrow cells in response to inflammation, hyperglycemia, and growth hormones. IL-1, IL-6, TNF-alpha as well as lipopolysaccharide (LPS) are strong inducers of resistin as observed in animal models of hepatic inflammation and necrosis. High levels of resistin are also observed in patients with pancreatitis and with systemic lupus erythematosus.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human Resistin Capture Antibody 1 x 600µl 10X Human Resistin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Resistin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. -
组织特异性
Expressed only in fatty tissues. -
序列相似性
Belongs to the resistin/FIZZ family. -
细胞定位
Secreted. - Information by UniProt
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别名
- Adipose tissue specific secretory factor
- Adipose tissue-specific secretory factor
- ADSF
see all -
数据库链接
- Entrez Gene: 56729 Human
- Omim: 605565 Human
- SwissProt: Q9HD89 Human
- Unigene: 283091 Human
图片
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Background-subtracted data values (mean +/- SD) are graphed
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The levels of native Resistin in serum samples were tested from ten individual healthy donors. Levels were interpolated from the standard curve in Sample Diluent NS. The mean level of Resistin was 2.6 ng/mL with a range of 1.6 to 5.3 ng/mL and a standard deviation of 1 ng/mL.
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Background-subtracted data values (mean +/- SD) are graphed.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab183364 被引用在 3 文献中.
- Mir MM et al. Differential Association of Selected Adipocytokines, Adiponectin, Leptin, Resistin, Visfatin and Chemerin, with the Pathogenesis and Progression of Type 2 Diabetes Mellitus (T2DM) in the Asir Region of Saudi Arabia: A Case Control Study. J Pers Med 12:N/A (2022). PubMed: 35629157
- Tang Y et al. A panel of urine-derived biomarkers to identify sepsis and distinguish it from systemic inflammatory response syndrome. Sci Rep 11:20794 (2021). PubMed: 34675320
- Metwalli O et al. Study of Some Inflammatory Mediators in the Serum of Patients With Atherosclerosis and Acute Myocardial Infarction. Cureus 13:e18450 (2021). PubMed: 34745775