重组Anti-Peroxiredoxin 5抗体[EPR14529(B)] (ab180587)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14529(B)] to Peroxiredoxin 5
- Suitable for: WB, IHC-P, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Peroxiredoxin 5抗体[EPR14529(B)]
参阅全部 Peroxiredoxin 5 一抗 -
描述
兔单克隆抗体[EPR14529(B)] to Peroxiredoxin 5 -
宿主
Rabbit -
特异性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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经测试应用
适用于: WB, IHC-P, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, HepG2 and A549 cell line lysates; Human kidney tissue; Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR14529(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180587于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 22 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
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IP |
1/50.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 22 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
IP
1/50. |
靶标
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功能
Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the peroxiredoxin 2 family.
Contains 1 thioredoxin domain. -
细胞定位
Mitochondrion. Cytoplasm. Peroxisome. - Information by UniProt
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数据库链接
- Entrez Gene: 25824 Human
- Entrez Gene: 54683 Mouse
- Entrez Gene: 113898 Rat
- Omim: 606583 Human
- SwissProt: P30044 Human
- SwissProt: P99029 Mouse
- SwissProt: Q9R063 Rat
- Unigene: 502823 Human
see all -
别名
- ACR1 antibody
- Alu co repressor 1 antibody
- Alu corepressor 1 antibody
see all
图片
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Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: PRDX5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)
Lane 4: A549 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab180587 observed at 17 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab180587 was shown to specifically react with PRDX5 when PRDX5 knockout samples were used. Wild-type and PRDX5 knockout samples were subjected to SDS-PAGE. Ab180587 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling Peroxiredoxin 5 with purified ab180587 at 1:500 dilution (1.18 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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ab180587 (Purified) at 1:30 dilution (2 µg) immunoprecipitating Peroxiredoxin 5 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2 (+): ab180587 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab180587 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
All lanes : Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/10000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Human brain lysates
Lane 3 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted? -
Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/5000 dilution (Purified) + Mouse brain lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted? -
Immunoprecipitation analysis of Peroxiredoxin 5 pulled down from HeLa cell lysates using ab180587 at 1/50 dilution.
Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/1000 dilution (Unpurified) + HeLa cell immunoprecipitated with ab180587 at 1/50.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Observed band size: 17 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible IgG) -
Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling Peroxiredoxin 5 with unpurified ab180587 at 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/5000 dilution (Unpurified)
Lane 1 : HepG2 cell line lysate
Lane 2 : Jurkat cell line lysate
Lane 3 : A549 cell line lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted? -
Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/20000 dilution (Unpurified) + HeLa cell line lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (6)
ab180587 被引用在 6 文献中.
- Cao X et al. ROS-mediated hypomethylation of PRDX5 promotes STAT3 binding and activates the Nrf2 signaling pathway in NSCLC. Int J Mol Med 47:573-582 (2021). PubMed: 33416106
- Queiroz RF et al. Hydrogen peroxide signaling via its transformation to a stereospecific alkyl hydroperoxide that escapes reductive inactivation. Nat Commun 12:6626 (2021). PubMed: 34785665
- Gao J et al. Metformin protects against PM2.5-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase a2. Redox Biol 28:101345 (2020). PubMed: 31669973
- Baković J et al. A key metabolic integrator, coenzyme A, modulates the activity of peroxiredoxin 5 via covalent modification. Mol Cell Biochem 461:91-102 (2019). PubMed: 31375973
- Brown LA et al. Late life maintenance and enhancement of functional exercise capacity in low and high responding rats after low intensity treadmill training. Exp Gerontol 125:110657 (2019). PubMed: 31306740
- Wang H et al. AMPKa2 deficiency exacerbates long-term PM2.5 exposure-induced lung injury and cardiac dysfunction. Free Radic Biol Med 121:202-214 (2018). PubMed: 29753072