Anti-Werner's syndrome helicase WRN抗体(ab17987)
Key features and details
- Rabbit polyclonal to Werner's syndrome helicase WRN
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Werner's syndrome helicase WRN抗体
参阅全部 Werner's syndrome helicase WRN 一抗 -
描述
兔多克隆抗体to Werner's syndrome helicase WRN -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa IP: HeLa
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab17987于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at 2-5 µg/mg of lysate.
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WB | (2) |
1/5000 - 1/25000. Predicted molecular weight: 162 kDa.
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说明 |
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IP
Use at 2-5 µg/mg of lysate. |
WB
1/5000 - 1/25000. Predicted molecular weight: 162 kDa. |
靶标
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功能
Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A. -
疾病相关
Defects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.
Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:114500]. -
序列相似性
Belongs to the helicase family. RecQ subfamily.
Contains 1 3'-5' exonuclease domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain. -
翻译后修饰
Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus > nucleolus. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7486 Human
- Omim: 604611 Human
- SwissProt: Q14191 Human
- Unigene: 632050 Human
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别名
- DKFZp686C2056 antibody
- DNA helicase antibody
- DNA helicase, RecQ like type 3 antibody
see all
图片
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All lanes : Anti-Werner's syndrome helicase WRN antibody (ab17987) at 1/5000 dilution
Lane 1 : Hela Whole cell lysate at 50 µg
Lane 2 : Hela Whole cell lysate at 15 µg
Lane 3 : Hela Whole cell lysate at 5 µg
Predicted band size: 162 kDaDetection: Chemiluminescence with exposure time of 30 second
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Werner's syndrome helicase WRN was immunoprecipitated from HeLa whole cell lysate with 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab17987 at 1 µg/ml dilution.
Lane 1: ab17987 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab17987 被引用在 1 文献中.
- Otterlei M et al. Werner syndrome protein participates in a complex with RAD51, RAD54, RAD54B and ATR in response to ICL-induced replication arrest. J Cell Sci 119:5137-46 (2006). WB, IP ; Human . PubMed: 17118963