重组Anti-Von Willebrand Factor抗体[EPR12010] (ab179451)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12010] to Von Willebrand Factor
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-Von Willebrand Factor抗体[EPR12010]
参阅全部 Von Willebrand Factor 一抗 -
描述
兔单克隆抗体[EPR12010] to Von Willebrand Factor -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Von Willebrand Factor aa 1200-1300. The exact sequence is proprietary.
Database link: P04275 -
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12010 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab179451于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000. Predicted molecular weight: 309 kDa.
|
|
IHC-P |
1/8000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
---|
WB
1/1000. Predicted molecular weight: 309 kDa. |
IHC-P
1/8000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. -
组织特异性
Plasma. -
疾病相关
Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII. -
序列相似性
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 4 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFA domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains. -
结构域
The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules. -
翻译后修饰
All cysteine residues are involved in intrachain or interchain disulfide bonds.
N- and O-glycosylated. -
细胞定位
Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules. - Information by UniProt
-
数据库链接
- Entrez Gene: 7450 Human
- Omim: 613160 Human
- SwissProt: P04275 Human
- Unigene: 440848 Human
-
别名
- Coagulation factor VIII antibody
- Coagulation factor VIII VWF antibody
- F8VWF antibody
see all
图片
-
Anti-Von Willebrand Factor antibody [EPR12010] (ab179451) at 1/1000 dilution (Purified) + Human plasma lysate
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 309 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling Von Willebrand Factor with Purified ab179451 at 1:8000 (0.12 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric cancer tissue sections labeling Von Willebrand Factor with Purified ab179451 at 1:8000 (0.12 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab179451 被引用在 3 文献中.
- Rutkai I et al. Neuropathology and virus in brain of SARS-CoV-2 infected non-human primates. Nat Commun 13:1745 (2022). PubMed: 35365631
- Dou X et al. Fibrotic and inflammatory characteristics of epidural fat adjacent to the ossification area in patients with ossification of the ligament flavum. JOR Spine 5:e1229 (2022). PubMed: 36601380
- Aid M et al. Vascular Disease and Thrombosis in SARS-CoV-2-Infected Rhesus Macaques. Cell 183:1354-1366.e13 (2020). PubMed: 33065030