重组人CCNO蛋白(Active) (ab177582)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: proprietary tag N-Terminus, proprietary tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, WB
描述
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产品名称
重组人CCNO蛋白(Active)
参阅全部 CCNO 蛋白酶 -
生物活性
The specific activity of Cdk2/ Cyclin O was determined to be 46 nmol/min/mg.
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纯度
> 90 % SDS-PAGE.
Assessed by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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氨基酸序列 1
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种属
Human -
序列
MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIR EISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIP LPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLAR AFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRR ALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSK VVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL -
预测分子量
58 kDa including tags -
氨基酸
1 to 298 -
标签
proprietary tag N-Terminus -
额外的序列信息
NM_001798
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氨基酸序列 2
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种属
Human -
序列
MVTPCPTSPSSPAARAGRRDNDQNLRAPVKKSRRPRLRRKQPLHPLNPCP LPGDSGICDL FESPSSGSDGAESPSAARGGSPLPGPAQPVAQLDLQTF RDYGQSCYAFRKAQESHFHPRE ALARQPQVTAESRCKLLSWLIPVHRQ FGLSFESLCLTVNTLDRFLTTTPVAADCFQLLGV TSLLIACKQVEVHP PRVKQLLALCCGAFSRQQLCNLECIVLHKLHFTLGAPTISFFLEHF TH ARVEAGQAEASEALEAQALARGVAELSLADYAFTSYSPSLLAICCLALAD RMLRVSRP VDLRLGDHPEAALEDCMGKLQLLVAINSTSLTHMLPVQIC EKCSLPPSSK -
预测分子量
68 kDa including tags -
氨基酸
1 to 350 -
标签
proprietary tag N-Terminus -
额外的序列信息
NM_021147
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技术指标
Our Abpromise guarantee covers the use of ab177582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
Western blot
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形式
Liquid -
补充说明
ab89813 (Histone H1 protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris-HCl buffer, 0.88% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- CCNO
- CCNO_HUMAN
- CCNU
see all -
序列相似性
Belongs to the cyclin family. -
发展阶段
Maximum levels during G(1) phase. Levels decrease through S and G(2) phases. - Information by UniProt
图片
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The specific activity of CCNO (ab177582) was determined to be 43.8 nmol/min/mg as per activity assay protocol
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SDS PAGE analysis of ab177582
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The specific activity of Cdk2/ Cyclin O was determined to be 46 nmol/min/mg.
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SDS-PAGE analysis of ab177852 which was determined to be >90% pure by densitometry. Cdk2 is approximately 58 kDa and Cyclin O is approximately 68 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab177582 尚未被引用在任何文献中。