Apoptosis/ Necrosis Assay试剂盒(blue,green,red) (ab176749)
Key features and details
- Assay type: Cell-based
- Platform: Flow cytometer, Fluorescence microscope
- Assay time: 1 hr
- Sample type: Adherent cells, Suspension cells
概述
-
产品名称
Apoptosis/ Necrosis Assay试剂盒(blue,green,red)
参阅全部 Apoptosis/ Necrosis 试剂盒 -
样品类型
Adherent cells, Suspension cells -
检测类型
Cell-based -
检测时间
1h 00m -
产品概述
Apoptosis/ Necrosis Detection Kit (blue, green, red) (ab176749) is designed to simultaneously monitor apoptotic, necrotic and healthy cells.
The PS sensor used in this kit has green fluorescence (Ex/Em = 490/525 nm) upon binding to membrane PS.
Necrosis has been characterized as passive, accidental cell death resulting from environmental perturbations with uncontrolled release of inflammatory cellular contents.
Loss of plasma membrane integrity, as demonstrated by the ability of a membrane-impermeable 7-AAD (Ex/Em = 546/647 nm) to label the nucleus, represents a straightforward approach to demonstrate late stage apoptosis and necrosis.
In addition, this kit also provides a live cell cytoplasm labeling dye, CytoCalcein Violet 450 (Ex/Em = 405/450 nm), for labeling living cell cytoplasm.
This kit is optimized to simultaneously detect cell apoptosis (green), necrosis (green and/or red) and healthy cells (blue) with a flow cytometer or fluorescence microscope.
This product was previously called Apoptosis/ Necrosis Detection Kit.
-
说明
For more apoptosis assays, review the apoptosis assay and apoptosis marker guide.
-
平台
Flow cytometer, Fluorescence microscope
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests 200X 7-AAD 1 x 100µl Apopxin Green Indicator 1 x 200µl Assay Buffer 1 x 50ml CytoCalcein Violet 450 1 vial -
研究领域
图片
-
Fluorescent analysis showing cells that are live (blue, stained by CytoCalcein Violet 450), apoptotic (green, Apopxin Green Indicator), and necrotic (red, indicated by 7-AAD staining) in Jurkat cells induced by 1μM staurosporine for 3 hours. The fluorescence images of the cells were taken with a fluorescent microscope through the Violet, FITC and TRITC channel respectively. Individual images taken from each channel from the same cell population were merged as shown.
-
Fluorescent analysis of live non-induced Jurkat cells stained by CytoCalcein Violet 450.
-
Jurkat cells were treated without (Blue) or with 1 μM staurosporine (Red) in a 37 oC, 5% CO2 incubator for 5 hours, and then
loaded with Apopxin Green Indicator for 30 minutes. The fluorescence intensity of Apopxin Green Indicator was measured with a
flow cytometer using FL1 channel.
数据表及文件
-
SDS download
-
Datasheet download
文献 (67)
ab176749 被引用在 67 文献中.
- Tregub P et al. Permissive hypercapnia and hypercapnic hypoxia inhibit signaling pathways of neuronal apoptosis in ischemic/hypoxic rats. Mol Biol Rep 50:2317-2333 (2023). PubMed: 36575322
- Maurici CE et al. Hyperthermia Enhances Efficacy of Chemotherapeutic Agents in Pancreatic Cancer Cell Lines. Biomolecules 12:N/A (2022). PubMed: 35625581
- Martin TG et al. Pharmacological inhibition of BAG3-HSP70 with the proposed cancer therapeutic JG-98 is toxic for cardiomyocytes. J Cell Biochem 123:128-141 (2022). PubMed: 34487557
- Pallagi P et al. Bile acid- and ethanol-mediated activation of Orai1 damages pancreatic ductal secretion in acute pancreatitis. J Physiol 600:1631-1650 (2022). PubMed: 35081662
- Zerfaoui M et al. Nuclear Localization of BRAFV600E Is Associated with HMOX-1 Upregulation and Aggressive Behavior of Melanoma Cells. Cancers (Basel) 14:N/A (2022). PubMed: 35053476