Fixable Cell Viability Assay试剂盒(Fluorometric - Blue) - Cytopainter (ab176741)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Flow cytometer
- Sample type: Adherent cells, Suspension cells
概述
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产品名称
Fixable Cell Viability Assay试剂盒(Fluorometric - Blue) - Cytopainter
参阅全部 Cell Viability 试剂盒 -
检测方法
Fluorescent -
样品类型
Adherent cells, Suspension cells -
检测类型
Cell-based (quantitative) -
种属反应性
与反应: Mammals, Other species -
产品概述
Abcam's Fixable Cell Viability Assay Kit (Fluorometric - Blue) | Cytopainter (ab176741) is used to evaluate the viability of mammalian cells by flow cytometry. The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 folds and can be completely preserved after fixation.
Fixable Cell Viability Assay Kit (Fluorometric - Blue) | Cytopainter (ab176741) is designed to label cells at Ex/ Em =353/442 nm excited with a laser at 335 nm.
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说明
Related assays
Review the cell health assay guide to learn about kits to perform a cell viability assay, cytotoxicity assay and cell proliferation assay.
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平台
Flow cytometer
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 200 tests DMSO 1 x 200µl Tracking dye Blue 1 vial -
研究领域
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相关性
Cell viability is a determination of living or dead cells, based on a total cell population. Cell viability assess healthy cells in a sample, with no distinction between dividing or quiescent cells. An increase in cell viability indicates cell growth, while a decrease in viability can generally be interpreted as the result of either toxic effects of compounds/agents or suboptimal culture conditions. -
别名
- cell tracking
图片
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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