重组Anti-DOCK8抗体[EPR12511] (ab175208)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12511] to DOCK8
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-DOCK8抗体[EPR12511]
参阅全部 DOCK8 一抗 -
描述
兔单克隆抗体[EPR12511] to DOCK8 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Recombinant fragment within Human DOCK8. The exact sequence is proprietary.
Database link: Q8NF50 -
阳性对照
- Raji and THP1 cell lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12511 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175208于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/10000 - 1/50000. Predicted molecular weight: 239 kDa.
|
|
ICC/IF |
1/500.
For unpurified use at 1/100 - 1/250 |
说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Predicted molecular weight: 239 kDa. |
ICC/IF
1/500. For unpurified use at 1/100 - 1/250 |
靶标
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功能
Potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP. -
疾病相关
Defects in DOCK8 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal recessive (AR-HIES) [MIM:243700]. It is a rare disorder of immunity characterized by immunodeficiency, recurrent infections, eczema, increased serum IgE, eosinophilia and lack of connective tissue and skeletal involvement. -
序列相似性
Belongs to the DOCK family.
Contains 1 DHR-1 (CZH-1) domain.
Contains 1 DHR-2 (CZH-2) domain. -
结构域
The DHR-2 domain may mediate some GEF activity. - Information by UniProt
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数据库链接
- Entrez Gene: 81704 Human
- Omim: 611432 Human
- SwissProt: Q8NF50 Human
- Unigene: 132599 Human
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别名
- 1200017A24Rik antibody
- Dedicator of cytokinesis protein 8 antibody
- DOCK8 antibody
see all
图片
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Immunocytochemistry/Immunofluorescence analysis of Raji (human Burkitt's lymphoma) cells labelling DOCK8 with purified ab175208 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
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All lanes : Anti-DOCK8 antibody [EPR12511] (ab175208) at 1/10000 dilution
Lane 1 : Raji cell lysate
Lane 2 : THP1 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 239 kDa -
Immunofluorescent staining of Raji cells labeling DOCK8 with ab175208 at 1/100 dilution (green). DAPI nuclear staining (blue).
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Intracellular flow cytometric analysis of permeabilized THP1 cells labeling DOCK8with ab175208at 1/10 dilution (red) compared to a Rabbit IgG (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab175208 被引用在 4 文献中.
- Meshaal SS et al. Diagnosis of DOCK8 deficiency using Flow cytometry Biomarkers: an Egyptian Center experience. Clin Immunol 195:36-44 (2018). PubMed: 30048691
- Cao M et al. Mechanisms of Impaired Neutrophil Migration by MicroRNAs in Myelodysplastic Syndromes. J Immunol 198:1887-1899 (2017). PubMed: 28130497
- Qin T et al. Novel DOCK8 gene mutations lead to absence of protein expression in patients with hyper-IgE syndrome. Immunol Res N/A:N/A (2015). PubMed: 26659092
- Kruithof-de Julio M et al. Canonical Wnt signaling regulates Nkx3.1 expression and luminal epithelial differentiation during prostate organogenesis. Dev Dyn 242:1160-71 (2013). PubMed: 23813564