重组Anti-QPRT抗体[EPR11941(B)] (ab171944)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11941(B)] to QPRT
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-QPRT抗体[EPR11941(B)]
参阅全部 QPRT 一抗 -
描述
兔单克隆抗体[EPR11941(B)] to QPRT -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF,IHC or IP -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Synthetic peptide within Human QPRT aa 250 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: Q15274 -
阳性对照
- Human fetal kidney, Human fetal liver and Human fetal brain lysate, HepG2 and HeLa cell line lysates, Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR11941(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab171944于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/5000. Predicted molecular weight: 31 kDa.
|
说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Predicted molecular weight: 31 kDa. |
靶标
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功能
Involved in the catabolism of quinolinic acid (QA). -
通路
Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1. -
序列相似性
Belongs to the nadC/modD family. - Information by UniProt
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数据库链接
- Entrez Gene: 23475 Human
- Omim: 606248 Human
- SwissProt: Q15274 Human
- Unigene: 513484 Human
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别名
- NADC_HUMAN antibody
- Nicotinate nucleotide pyrophosphorylase (carboxylating) antibody
- Nicotinate-nucleotide pyrophosphorylase [carboxylating] antibody
see all
图片
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: QPRT knockout HAP1 cell lysate (20 µg)
Lane 3: HepG2 cell lysate (20 µg)
Lane 4: Human fetal brain cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab171944 observed at 35 kDa. Red - loading control, ab8245, observed at 37 kDa.ab171944 was shown to specifically react with QPRT when QPRT knockout samples were used. Wild-type and QPRT knockout samples were subjected to SDS-PAGE. ab171944 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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All lanes : Anti-QPRT antibody [EPR11941(B)] (ab171944) at 1/1000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : Jurkat cell lysate
Lane 6 : Human fetal heart tissue lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 31 kDa -
Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling QPRTwith ab171944 at 1/10 (red)or a rabbit IgG (negative) (green).
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab171944 被引用在 6 文献中.
- Fahrmann JF et al. Mutational Activation of the NRF2 Pathway Upregulates Kynureninase Resulting in Tumor Immunosuppression and Poor Outcome in Lung Adenocarcinoma. Cancers (Basel) 14:N/A (2022). PubMed: 35626147
- Li T et al. Co-treatment With Everolimus, an mTOR-Specific Antagonist, or Downregulation of ELK1 Enhances the Sensitivity of Pancreatic Cancer Cells to Genistein. Front Cell Dev Biol 9:633035 (2021). PubMed: 34540820
- Zhou XY et al. Copy Number Variations Analysis Identifies QPRT as a Candidate Gene Associated With Susceptibility for Solitary Functioning Kidney. Front Genet 12:575830 (2021). PubMed: 34079576
- Kudo K et al. Divergent metabolic responses dictate vulnerability to NAMPT inhibition in ovarian cancer. FEBS Lett 594:1379-1388 (2020). PubMed: 31950503
- Niu YC et al. MicroRNA-654-3p enhances cisplatin sensitivity by targeting QPRT and inhibiting the PI3K/AKT signaling pathway in ovarian cancer cells. Exp Ther Med 20:1467-1479 (2020). PubMed: 32742380
- Yue Z et al. Silencing DSCAM-AS1 suppresses the growth and invasion of ER-positive breast cancer cells by downregulating both DCTPP1 and QPRT. Aging (Albany NY) 12:14754-14774 (2020). PubMed: 32716908