人Inflammation抗体阵列-膜(20 targets) (ab169804)
概述
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产品名称
人Inflammation抗体阵列-膜(20 targets)
参阅全部 Inflammation Antibody Array 抗体芯片 -
样品类型
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media -
检测类型
Semi-quantitative -
种属反应性
与反应: Human -
产品概述
ab169804 detects 20 Human Inflammatory Factors in 4 or 8 samples. Suitable for all liquid sample types.
Targets: Eotaxin-1, Eotaxin-2, G-CSF, GM-CSF, IFN-gamma, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, I-309, TIMP-2
Learn more about membrane antibody arrays
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说明
If you are interested in this cytokine array, arrays ab133996, ab133997, ab169817, ab133998 and ab134003 may also be of interest. A list of human antibody membrane arrays is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Multiplex Protein Detectionmore details
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 4 Membranes 1 x 8 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1X Blocking Buffer 1 x 25ml 2 x 25ml 2000X Biotin-Conjugated Anti Cytokines 2 vials 4 vials 20X Wash Buffer I 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit Detection Buffer C 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml Inflammation Antibody Array Membranes 4 units 8 units -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab169804于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Multiplex Protein Detection |
Use at an assay dependent concentration.
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说明 |
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Multiplex Protein Detection
Use at an assay dependent concentration. |
图片
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Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate.
Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Media alone was used as a negative control.
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Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Media alone was used as a negative control. Mean pixel density was quantified using CCD camera software analysis.
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HepG2 cells (Human hepatocellular carcinoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804.
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Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Mean pixel density was quantified using CCD camera software analysis.
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Typical results obtained with Abcam Membrane Antibody Arrays. Image above presents typical images obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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