Anti-c-Myb抗体[ANA236B] (ab169111)
Key features and details
- Rat monoclonal [ANA236B] to c-Myb
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-c-Myb抗体[ANA236B]
参阅全部 c-Myb 一抗 -
描述
大鼠单克隆抗体[ANA236B] to c-Myb -
宿主
Rat -
经测试应用
适用于: WBmore details
不适用于: ICC,IHC-Fr,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Cow -
免疫原
Recombinant fragment (His-tag) corresponding to Human c-Myb aa 402-640.
Sequence:FLNTSSNHE NSDLEMPSLT STPLIGHKLT VTTPFHRDQT VKTQKENTVF RTPAIKRSIL ESSPRTPTPF KHALAAQEIK YGPLKMLPQT PSHLVEDLQD VIKQESDESG IVAEFQENGP PLLKKIKQEV ESPTDKSGNF FCSHHWEGDS LNTQLFTQTS PVADAPNILT SSVLMAPASE DEDNVLKAFT VPKNRSLASP LQPCSSTWEP ASCGKMEEQM TSSSQARKYV NAFSARTLVM
Database link: P10242 -
阳性对照
- Recombinant Human c-Myb protein (ab114406) can be used as a positive control in WB. Hek-V5-c-Myb transfected cells; KARPAS299, Jurkat, MT2 and SUP-T1 cell lysates; Human thymus, bone marrow and lymphoblastic lymphoma tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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克隆
单克隆 -
克隆编号
ANA236B -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab169111于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/50. Predicted molecular weight: 72 kDa.
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说明 |
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WB
1/50. Predicted molecular weight: 72 kDa. |
靶标
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功能
Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. -
序列相似性
Contains 3 HTH myb-type DNA-binding domains. -
结构域
Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression. -
翻译后修饰
Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 317776 Cow
- Entrez Gene: 4602 Human
- Entrez Gene: 17863 Mouse
- Omim: 189990 Human
- SwissProt: P46200 Cow
- SwissProt: P10242 Human
- SwissProt: P06876 Mouse
- Unigene: 606320 Human
see all -
别名
- C myb antibody
- c myb protein (140 AA) antibody
- c-myb protein (140 AA) antibody
see all
图片
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: c-Myb knockout HAP1 whole cell lysate (20 µg)Lanes 1 - 2: Merged signal (red and green). Green - ab169111 observed at 75 kDa. Red - loading control, ab181602, observed at 37 kDa.
Ab169111 was shown to specifically react with c-Myb, in wild type HAP1 cells as signal was lost in c-Myb knockout cells. Wild-type and c-Myb knockout samples were subjected SDS-PAGE. Ab169111 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/50 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) (ab253031) preabsorbed and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-c-Myb antibody [ANA236B] (ab169111) at 1/50 dilution
Lane 1 : DAUDI cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : Raji cell lysate
Lane 4 : KARPAS299 cell lysate
Lane 5 : HUT78 cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : MT2 cell lysate
Lane 8 : SUP-T1 cell lysate
Lane 9 : YT cell lysate
Lysates/proteins at 150 µg per lane.
Predicted band size: 72 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab169111 尚未被引用在任何文献中。