鸡IgY ELISA试剂盒(ab157693)
Key features and details
- Sensitivity: 1.5305 ng/ml
- Range: 6.25 ng/ml - 400 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Chicken
概述
-
产品名称
鸡IgY ELISA试剂盒
参阅全部 IgY 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 10% -
样品类型
Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
1.5305 ng/ml -
范围
6.25 ng/ml - 400 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum > 85 % - % -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Chicken -
产品概述
Abcam’s IgY Chicken ELISA kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for the quantitative measurement of IgY in chicken serum or plasma samples.
In this assay the IgY present in samples reacts with the anti-IgY antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti- IgY antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgY. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgY in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgY in the test sample. The quantity of IgY in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Get results in 90 minutes with Chicken IgY ELISA Kit (ab189577) from our SimpleStep ELISA® range.
-
平台
Microplate
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 100X HRP-conjugated anti-chicken IgY antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chicken IgY Calibrator (Lyophilized) 1 vial Chicken IgY ELISA Microplate 1 unit Chromogen Substrate Solution 1 x 12ml Stop Solution 1 x 12ml -
研究领域
-
相关性
Secreted immunoglobulins have two separable functions: one is to bind specifically to pathogenic molecules, the other is to recruit cells and molecules to destroy the opsonized pathogen. IgY has a tetrameric structure consisting of two identical light chains (Mr = 24 kDa) and two identical heavy chains (Mr = 73 kDa). -
别名
- FCR
- IGSF1
- immunoglobulin superfamily, member 1
-
数据库链接
- Entrez Gene: 419114 Chicken
相关产品
-
SimpleStep ELISA kits
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab157693 被引用在 2 文献中.
- Lee KY et al. Development and characterization of a CRISPR/Cas9-mediated RAG1 knockout chicken model lacking mature B and T cells. Front Immunol 13:892476 (2022). PubMed: 36032098
- Wang WC et al. Supplementation of Bacillus subtilis-based probiotic reduces heat stress-related behaviors and inflammatory response in broiler chickens. J Anim Sci 96:1654-1666 (2018). PubMed: 29528406