Anti-Lysosomal acid lipase/LAL抗体(ab154356)
Key features and details
- Rabbit polyclonal to Lysosomal acid lipase/LAL
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Lysosomal acid lipase/LAL抗体
参阅全部 Lysosomal acid lipase/LAL 一抗 -
描述
兔多克隆抗体to Lysosomal acid lipase/LAL -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human Lysosomal acid lipase/LAL aa 164-399.
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阳性对照
- A549 whole cell lysate, H1299 whole cell lysate, mouse liver lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab154356于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa. |
靶标
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功能
Crucial for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor-mediated endocytosis of lipoprotein particles. Important in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation. -
疾病相关
Defects in LIPA are the cause of Wolman disease (WOD) [MIM:278000]. WOD is a severe manifestation of LIPA deficiency, leading to the accumulation of cholesteryl esters and triglycerides in most tissues of the body. WOD occurs in infancy and is nearly always fatal before the age of 1 year.
Defects in LIPA are the cause of cholesteryl ester storage disease (CESD) [MIM:278000]. CESD is a mild manifestation of LIPA deficiency, leading to the accumulation of cholesteryl esters and triglycerides in most tissues of the body. It is characterized by late-onset. -
序列相似性
Belongs to the AB hydrolase superfamily. Lipase family. -
细胞定位
Lysosome. - Information by UniProt
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数据库链接
- Entrez Gene: 3988 Human
- Entrez Gene: 16889 Mouse
- Omim: 278000 Human
- SwissProt: P38571 Human
- SwissProt: Q9Z0M5 Mouse
- Unigene: 643030 Human
- Unigene: 157545 Mouse
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别名
- Acid cholesteryl ester hydrolase antibody
- CESD antibody
- cholesterol ester hydrolase antibody
see all
图片
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Anti-Lysosomal acid lipase/LAL antibody (ab154356) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 45 kDa
10% SDS PAGE -
All lanes : Anti-Lysosomal acid lipase/LAL antibody (ab154356) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
10% SDS PAGE
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab154356 被引用在 4 文献中.
- Carotti S et al. Molecular and histological traits of reduced lysosomal acid lipase activity in the fatty liver. Cell Death Dis 12:1092 (2021). PubMed: 34795230
- Wang H et al. An AMPK-dependent, non-canonical p53 pathway plays a key role in adipocyte metabolic reprogramming. Elife 9:N/A (2020). PubMed: 33320092
- Wang C et al. Autophagic lipid metabolism sustains mTORC1 activity in TSC-deficient neural stem cells. Nat Metab 1:1127-1140 (2019). PubMed: 32577608
- Llewellyn KJ et al. A Fine Balance of Dietary Lipids Improves Pathology of a Murine Model of VCP-Associated Multisystem Proteinopathy. PLoS One 10:e0131995 (2015). WB ; Mouse . PubMed: 26134519