SN 38,DNA topoisomerase I抑制剂(ab141108)
Key features and details
- DNA topoisomerase I inhibitor. Active metabolite of CPT-11.
- CAS Number: 86639-52-3
- Purity: > 99%
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
SN 38,DNA topoisomerase I抑制剂 -
描述
DNA topoisomerase I抑制剂. Active metabolite of CPT-11. -
别名
- 7-Ethyl-10-hydroxycamptothecin
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生物学描述
Active metabolite of CPT-11 (Irinotecan) (ab141107). Inhibits DNA topoisomerase I (IC50 values are 0.74 and 1.9 μM in P388 and Ehrlich cells respectively) and DNA and RNA synthesis (IC50 values are 77 nM and 1.3 μM respectively). Shows potent antitumor activity, causes S- and G2 cell cycle arrest and induces apoptosis. Active in vivo.
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纯度
> 99% -
CAS编号
86639-52-3 -
化学结构
性能
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化学名称
(4S)-4,11-Diethyl-4,9-dihydroxy-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione -
分子量
392.41 -
分子式
C22H20N2O5 -
PubChem识别号
104842 -
存放说明
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in DMSO to 100 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC[C@]1(O)C=2C=C3c4nc5ccc(O)cc5c(CC)c4CN3C(=O)C=2COC1=O -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab141108于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Functional Studies |
Use at an assay dependent concentration.
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说明 |
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Functional Studies
Use at an assay dependent concentration. |
图片
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2D chemical structure image of ab141108, SN 38, DNA topoisomerase I inhibitor
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ab2893 staining γH2A.X in HeLa cells treated with SN 38 (ab141108), by ICC/IF. Increase of γH2A.X nuclear expression correlates with increased concentration of SN 38, as described in literature.
The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab141108 (SN 38) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2893 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (4)
ab141108 被引用在 4 文献中.
- Tsai CL et al. Topoisomerase I Inhibition Radiosensitizing Hepatocellular Carcinoma by RNF144A-mediated DNA-PKcs Ubiquitination and Natural Killer Cell Cytotoxicity. J Clin Transl Hepatol 11:614-625 (2023). PubMed: 36969901
- Amin N et al. TP5: A Novel Therapeutic Approach Targeting Aberrant and Hyperactive CDK5/p25 for the Treatment of Colorectal Carcinoma. Int J Mol Sci 24:N/A (2023). PubMed: 37511490
- Winkler C et al. SLFN11 informs on standard of care and novel treatments in a wide range of cancer models. Br J Cancer 124:951-962 (2021). PubMed: 33339894
- Peter S et al. Cell-based high-throughput compound screening reveals functional interaction between oncofetal HMGA2 and topoisomerase I. Nucleic Acids Res N/A:N/A (2016). PubMed: 27587582