Glutamate Assay试剂盒(Fluorometric) (ab138883)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue, Urine
- Sensitivity: 1 µM
概述
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产品名称
Glutamate Assay试剂盒(Fluorometric)
参阅全部 Glutamate 试剂盒 -
检测方法
Fluorescent -
样品类型
Urine, Serum, Plasma, Other biological fluids, Tissue, Cell Lysate -
检测类型
Quantitative -
灵敏度
1 µM -
检测时间
0h 30m -
产品概述
Glutamate Assay Kit (Fluorometric) (ab138883) provides a quick and sensitive method for the measurement of glutamate (glutamic acid) in various biological samples.
In the glutamate assay protocol, the coupled enzyme system catalyzes the reaction between L-Glutamic acid and NADP+ to produce NADPH, which is specifically recognized by the NADPH sensor and recycled back to NADP+. During the reaction, a red fluorescence product is produced, which in turn can be detected in a fluorescence microplate reader at Ex/Em = 540/590 nm (range Ex/Em = 530 – 570/590 – 600 nm).
This assay can detect as little as 1 µM glutamic acid. The signal can also be read by absorbance at OD: 576 ± 5 nm, although the sensitivity of the assay is reduced 10-fold.
The assay is robust, and can be easily adapted to automation without separation step as no wash step is required.
Glutamate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min - 2 hr
- analyze with a microplate reader -
平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 200 tests Assay Buffer 1 x 10ml Dilution Buffer 1 x 10ml Enzyme Mix 1 unit Glutamic Acid 1 vial NADP 1 vial -
研究领域
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相关性
Glutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen. Glutamate is the most abundant swift excitatory neurotransmitter in the mammalian nervous system. It is believed to be involved in learning and memory and has appeared to be involved in diseases like amyotrophic lateral sclerosis, lathyrism, autism, some forms of mental retardation and Alzheimer's disease. Glutamic acid is also present in a wide variety of foods, and has been used as a flavor enhancer in food industry.
相关产品
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图片
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The concentration of intracellular glutamate in cells, during the exponential phase, grown at different hydrostatic pressures.
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Standard curve (30 minutes incubation): mean of duplicates (+/- SD), with background reads subtracted.
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Glutamic acid measured in mouse tissue lysates, showing quantity (nmol) per mg of extracted protein (mean of duplicates, +/- SD).
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Glutamic acid measured in cell lysates showing quantity (nmol) per 106 cells (mean of duplicates, +/- SD).
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Glutamate measured in human biological fluids (mean of duplicates, +/- SD).
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab138883 被引用在 14 文献中.
- Shrivastava V et al. Glutamate Uptake Is Not Impaired by Hypoxia in a Culture Model of Human Fetal Neural Stem Cell-Derived Astrocytes. Genes (Basel) 13:N/A (2022). PubMed: 35328060
- Piepgras J et al. Enhancement of Phosphorylation and Transport Activity of the Neuronal Glutamate Transporter Excitatory Amino Acid Transporter 3 by C3bot and a 26mer C3bot Peptide. Front Cell Neurosci 16:860823 (2022). PubMed: 35783090
- Pediaditakis I et al. A microengineered Brain-Chip to model neuroinflammation in humans. iScience 25:104813 (2022). PubMed: 35982785
- Jones-Muhammad M et al. Increased seizure sensitivity in pregnant mice with genetic knockdown of acid sensing ion channel 2a is associated with impaired hippocampal inflammatory response. Front Physiol 13:983506 (2022). PubMed: 36187797
- Wu B et al. ADSCs-derived exosomes ameliorate hepatic fibrosis by suppressing stellate cell activation and remodeling hepatocellular glutamine synthetase-mediated glutamine and ammonia homeostasis. Stem Cell Res Ther 13:494 (2022). PubMed: 36195966