Anti-Glutathione Peroxidase 2/GPX2抗体(ab137431)
Key features and details
- Rabbit polyclonal to Glutathione Peroxidase 2/GPX2
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Glutathione Peroxidase 2/GPX2抗体
参阅全部 Glutathione Peroxidase 2/GPX2 一抗 -
描述
兔多克隆抗体to Glutathione Peroxidase 2/GPX2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Dog, Pig, Monkey -
免疫原
Synthetic peptide corresponding to Human Glutathione Peroxidase 2/GPX2 aa 126-190.
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阳性对照
- Recombinant Human Glutathione Peroxidase 2/GPX2 protein (ab116151) can be used as a positive control in WB. HepG2 whole cell lysate; Human gastric carcinoma and Mouse muscle tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137431于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 22 kDa.
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IHC-P |
1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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IP |
Use a concentration of 5 µg/ml.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 22 kDa. |
IHC-P
1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
IP
Use a concentration of 5 µg/ml. |
靶标
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功能
Could play a major role in protecting mammals from the toxicity of ingested organic hydroperoxides. Tert-butyl hydroperoxide, cumene hydroperoxide and linoleic acid hydroperoxide but not phosphatidycholine hydroperoxide, can act as acceptors. -
组织特异性
Mostly in liver and gastrointestinal tract, not found in heart or kidney. -
序列相似性
Belongs to the glutathione peroxidase family. -
细胞定位
Cytoplasm. Mainly cytoplasmic. - Information by UniProt
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数据库链接
- Entrez Gene: 2877 Human
- Entrez Gene: 14776 Mouse
- Entrez Gene: 29326 Rat
- Omim: 138319 Human
- SwissProt: P18283 Human
- SwissProt: Q9JHC0 Mouse
- SwissProt: P83645 Rat
- Unigene: 2704 Human
see all -
别名
- Gastrointestinal glutathione peroxidase antibody
- GI GPx antibody
- Glutathione peroxidase 2 (gastrointestinal) antibody
see all
图片
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Immunoprecipitation of Glutathione Peroxidase 2/GPX2 protein from HepG2 whole cell extracts using 5 μg of ab137431.
Western blot analysis was performed using ab137431. -
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labelling Glutathione Peroxidase 2/GPX2 with ab137431 at 1/500 dilution.
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Anti-Glutathione Peroxidase 2/GPX2 antibody (ab137431) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 22 kDa
12% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Mouse muscle tissue labelling Glutathione Peroxidase 2/GPX2 with ab137431 at 1/500 dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab137431 被引用在 14 文献中.
- Li D et al. Metallothionein MT1M Suppresses Carcinogenesis of Esophageal Carcinoma Cells through Inhibition of the Epithelial-Mesenchymal Transition and the SOD1/PI3K Axis. Mol Cells 44:267-278 (2021). PubMed: 33820882
- Ringuet MT et al. Analysis of Bioavailability and Induction of Glutathione Peroxidase by Dietary Nanoelemental, Organic and Inorganic Selenium. Nutrients 13:N/A (2021). PubMed: 33806211
- Cheng Q et al. Production and purification of homogenous recombinant human selenoproteins reveals a unique codon skipping event in E. coli and GPX4-specific affinity to bromosulfophthalein. Redox Biol 46:102070 (2021). PubMed: 34304108
- Jogo T et al. Expression of CD44 variant 9 induces chemoresistance of gastric cancer by controlling intracellular reactive oxygen spices accumulation. Gastric Cancer 24:1089-1099 (2021). PubMed: 33963958
- Li F et al. GPX2 silencing relieves epithelial-mesenchymal transition, invasion, and metastasis in pancreatic cancer by downregulating Wnt pathway. J Cell Physiol 235:7780-7790 (2020). PubMed: 31774184