Anti-Hsp90抗体(ab13495)
Key features and details
- Rabbit polyclonal to Hsp90
- Suitable for: ICC/IF, IHC-P, ICC, IHC-Fr, ELISA, IP, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human, Xenopus laevis
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Hsp90抗体
参阅全部 Hsp90 一抗 -
描述
兔多克隆抗体to Hsp90 -
宿主
Rabbit -
特异性
Detects 90kD proteins corresponding to the molecular mass of hsp90aß. -
经测试应用
适用于: ICC/IF, IHC-P, ICC, IHC-Fr, ELISA, IP, Flow Cyt, WBmore details -
种属反应性
与反应: Mouse, Rat, Human, Xenopus laevis -
免疫原
Full length protein corresponding to Human Hsp90.
Database link: P08238 -
阳性对照
- ICC: Heat Shocked Cervical cancer cell line (HeLa).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab13495于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF | (3) |
1/100.
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IHC-P | (1) |
Use at an assay dependent concentration.
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ICC | (1) |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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IP | (1) |
Use at an assay dependent concentration.
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Flow Cyt | (1) |
Use at an assay dependent concentration.
See Abreview. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB | (7) |
1/20000 - 1/40000. Detects a band of approximately 90 kDa (predicted molecular weight: 85 kDa).
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说明 |
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ICC/IF
1/100. |
IHC-P
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. See Abreview. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/20000 - 1/40000. Detects a band of approximately 90 kDa (predicted molecular weight: 85 kDa). |
靶标
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功能
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. -
序列相似性
Belongs to the heat shock protein 90 family. -
结构域
The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1. -
翻译后修饰
ISGylated.
S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1. -
细胞定位
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 3320 Human
- Entrez Gene: 3326 Human
- Entrez Gene: 15516 Mouse
- Entrez Gene: 15519 Mouse
- Entrez Gene: 299331 Rat
- Entrez Gene: 301252 Rat
- Omim: 140571 Human
- Omim: 140572 Human
see all -
别名
- Heat shock 86 kDa antibody
- Heat shock protein 90kDa alpha cytosolic class A member 1 antibody
- Heat shock protein 90kDa alpha cytosolic class B member 1 antibody
see all
图片
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All lanes : Anti-Hsp90 antibody (ab13495) at 1/10000 dilution (2 hours at room temperature)
Lane 1 : A431 with BSA for 30 minutes at room temperature
Lane 2 : A549 with BSA for 30 minutes at room temperature
Lane 3 : HCT116 with BSA for 30 minutes at room temperature
Lane 4 : Hela with BSA for 30 minutes at room temperature
Lane 5 : HEK293 with BSA for 30 minutes at room temperature
Lane 6 : HepG2 with BSA for 30 minutes at room temperature
Lane 7 : HL-60 with BSA for 30 minutes at room temperature
Lane 8 : HUVEC with BSA for 30 minutes at room temperature
Lane 9 : Jurkat with BSA for 30 minutes at room temperature
Lane 10 : MCF7 with BSA for 30 minutes at room temperature
Lane 11 : PC3 with BSA for 30 minutes at room temperature
Lane 12 : T98G with BSA for 30 minutes at room temperature
Lysates/proteins at 2 µg per lane.
Blocking peptides at 1.5 % per lane.
Secondary
All lanes : HRP Donkey anti-rabbit IgG, 1 hour at room temperature
Predicted band size: 85 kDa -
Anti-Hsp90 antibody (ab13495) at 1/1000 dilution + whole cell lysate prepared from human platelets treated with A23187 for 1 hour at 20 µg
Secondary
HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 85 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
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ab13495 staining Hsp90 in Mouse backskin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Bouin's fixative solution. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A FITC-conjugated Goat anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody.
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Immunofluorescent analysis of 2% paraformaldehyde fixed Heat Shocked Cervical cancer cell line (HeLa) for 20min at RT, labelling Hsp90 with ab13495 at 1/100 dilution for 12 hours at 4°C, followed by secondary antibody Goat Anti-Rabbit (FITC) (green) at 1/200 dilution for 2 hours at RT. Image showing cytoplasmic staining on HeLa cells. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 Antibody (green) (C) Composite image.
数据表及文件
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SDS download
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Datasheet download
文献 (56)
ab13495 被引用在 56 文献中.
- Niu L et al. Sericin inhibits MDA-MB-468 cell proliferation via the PI3K/Akt pathway in triple-negative breast cancer. Mol Med Rep 23:N/A (2021). PubMed: 33313947
- Casagrande BP et al. AMPK in the gut-liver-brain axis and its influence on OP rats in an HSHF intake and WTD rat model. Pflugers Arch 473:1199-1211 (2021). PubMed: 34075446
- Mesquita PHC et al. Effects of Resistance Training on the Redox Status of Skeletal Muscle in Older Adults. Antioxidants (Basel) 10:N/A (2021). PubMed: 33652958
- Chiang S et al. Mechanisms of impaired mitochondrial homeostasis and NAD+ metabolism in a model of mitochondrial heart disease exhibiting redox active iron accumulation. Redox Biol 46:102038 (2021). PubMed: 34416478
- Li K et al. Hsp90 interacts with Cdc37, is phosphorylated by PKA/PKC, and regulates Src phosphorylation in human sperm capacitation. Andrology 9:185-195 (2021). PubMed: 32656999