生物素Anti-Fc epsilon RI/FCER1A抗体[CRA2] (ab122915)
Key features and details
- Biotin Mouse monoclonal [CRA2] to Fc epsilon RI/FCER1A
- Suitable for: Flow Cyt, WB, ICC/IF
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
概述
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产品名称
生物素Anti-Fc epsilon RI/FCER1A抗体[CRA2]
参阅全部 Fc epsilon RI/FCER1A 一抗 -
描述
生物素小鼠单克隆抗体[CRA2] to Fc epsilon RI/FCER1A -
宿主
Mouse -
偶联物
Biotin -
经测试应用
适用于: Flow Cyt, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human Fc epsilon RI/FCER1A aa 50-200.
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阳性对照
- CHO/alpha beta gamma cells
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常规说明
[biotin]/[IgG] = 6.9
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Ion Exchange Chromatography -
纯化说明
The IgG fraction was purified from serum free culture medium of mouse hybridoma by propriety chromatography under mild conditions. Filter sterilized. -
克隆
单克隆 -
克隆编号
CRA2 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122915于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
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ICC/IF |
Use at an assay dependent concentration.
|
说明 |
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Flow Cyt
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. |
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines. -
序列相似性
Contains 2 Ig-like (immunoglobulin-like) domains. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2205 Human
- Omim: 147140 Human
- SwissProt: P12319 Human
- Unigene: 897 Human
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别名
- Fc epsilon RI alpha antibody
- Fc epsilon RI alpha chain antibody
- Fc epsilon RI alpha-chain antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab122915 被引用在 5 文献中.
- Takai T et al. Direct expression of the extracellular portion of human FcepsilonRIalpha chain as inclusion bodies in Escherichia coli. Biosci Biotechnol Biochem 65:79-85 (2001). PubMed: 11272849
- Goto T et al. Enhanced expression of the high-affinity receptor for IgE (Fc(epsilon)RI) associated with decreased numbers of Langerhans cells in the lesional epidermis of atopic dermatitis. J Dermatol Sci 27:156-61 (2001). PubMed: 11641054
- Takai T et al. Epitope analysis and primary structures of variable regions of anti-human FcepsilonRI monoclonal antibodies, and expression of the chimeric antibodies fused with human constant regions. Biosci Biotechnol Biochem 64:1856-67 (2000). PubMed: 11055388
- Hasegawa S et al. Functional expression of the high affinity receptor for IgE (FcepsilonRI) in human platelets and its' intracellular expression in human megakaryocytes. Blood 93:2543-51 (1999). PubMed: 10194433
- Hakimi J et al. The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding. J Biol Chem 265:22079-81 (1990). PubMed: 2148316