OcTMAB™,dynamin I and dynamin II抑制剂(ab120467)
Key features and details
- Cell-permeable dynamin I and dynamin II inhibitor
- CAS Number: 1120-02-1
- Soluble in DMSO to 25 mM (with warming)
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
OcTMAB™,dynamin I and dynamin II抑制剂 -
描述
Cell-permeable dynamin I and dynamin II抑制剂 -
生物学描述
Cell-permeable dynamin I and dynamin II inhibitor (IC50 values are 1.9 and 4.4 μM for inhibition of dynamin I and II GTPase, respectively). Targets the pleckstrin homology (PH) (lipid binding) domain. Competitive with phospholipid and non-competitive with GTP. Inhibits receptor-mediated endocytosis (IC50 = 6.7 μM). Inhibits cancer cell growth.
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常规说明
Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. OcTMAB™ is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd. -
CAS编号
1120-02-1 -
化学结构
性能
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化学名称
Octadecyltrimethylammonium bromide -
分子量
392.51 -
分子式
C21H46BrN -
PubChem识别号
70708 -
存放说明
Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in DMSO to 25 mM (with warming) -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
[Br-].C[N+](C)(C)CCCCCCCCCCCCCCCCCC -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120467于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Functional Studies |
Use at an assay dependent concentration.
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说明 |
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Functional Studies
Use at an assay dependent concentration. |
图片
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2D chemical structure image of ab120467, OcTMAB™, dynamin I and dynamin II inhibitor
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ab66705 staining PAI1 in HeLa cells treated with OcTMAB™ (ab120467), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of OcTMAB™, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120467 (OcTMAB™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (2)
ab120467 被引用在 2 文献中.
- Wang D et al. ATP Consumption Is Coupled with Endocytosis in Exudated Neutrophils. Int J Mol Sci 24:N/A (2023). PubMed: 37240384
- Wang D et al. ERK is involved in the differentiation and function of dimethyl sulfoxide-induced HL-60 neutrophil-like cells, which mimic inflammatory neutrophils. Int Immunopharmacol 84:106510 (2020). PubMed: 32361568