Anti-MyD88抗体[OTI1B4] (ab119048)
Key features and details
- Mouse monoclonal [OTI1B4] to MyD88
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Knockout validated
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
概述
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产品名称
Anti-MyD88抗体[OTI1B4]
参阅全部 MyD88 一抗 -
描述
小鼠单克隆抗体[OTI1B4] to MyD88 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Human MyD88.
Database link: Q99836 -
阳性对照
- WB: HEK293T cell lysate transfected with pCMV6-ENTRY MyD88 cDNA; Jerkat, and MOLT4 cell lysates. Flow Cyt (Intra): HEK293T cells transfected with pCMV6-ENTRY MyD88 overexpression plasmid; HeLa and Jurkat cells. ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY MyD88; HeLa and Jurkat cells.
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常规说明
The clone number has been updated from 1B4 to OTI1B4, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol, PBS -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant by affinity chromatography -
克隆
单克隆 -
克隆编号
OTI1B4 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119048于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/100.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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WB |
1/2000. Predicted molecular weight: 33 kDa.
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ICC/IF |
1/100.
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说明 |
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Flow Cyt (Intra)
1/100. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
1/2000. Predicted molecular weight: 33 kDa. |
ICC/IF
1/100. |
靶标
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功能
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. -
组织特异性
Ubiquitous. -
疾病相关
Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age. -
序列相似性
Contains 1 death domain.
Contains 1 TIR domain. -
结构域
The intermediate domain (ID) is required for the phosphorylation and activation of IRAK. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 4615 Human
- Omim: 602170 Human
- SwissProt: Q99836 Human
- Unigene: 82116 Human
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别名
- Mutant myeloid differentiation primary response 88 antibody
- MYD 88 antibody
- Myd88 antibody
see all
图片
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MyD88 knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: Molt-4 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab119048 observed at 37 kDa. Red - loading control, ab176560, observed at 52 kDa.ab119048 was shown to specifically react with MyD88 when MyD88 knockout samples were used. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. Ab119048 and ab176560 (loading control to alpha Tubulin) were diluted at 1/2000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 andGoat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-MyD88 antibody [OTI1B4] (ab119048) at 1/2000 dilution
Lane 1 : HEK293T lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T lysate transfected with pCMV6-ENTRY MyD88 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 33 kDa -
ab119048 at 1/100 dilution staining MyD88 in COS7 cells transiently transfected with pCMV6-ENTRY MyD88 by Immunofluorescence.
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ab119048 at 1/100 dilution, staining MyD88 in HEK293T cells transfected with pCMV6-ENTRY MyD88 overexpress plasmid(Red), or an empty vector control plasmid (Blue) by Flow Cytometry (Intracellular).
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ab119048 at 1/100 dilution staining MyD88 in HeLa cells by Flow cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).
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ab119048 at 1/100 dilution staining MyD88 in Jurkat cells by Flow cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab119048 被引用在 1 文献中.
- Xue X et al. Immunoregulatory Role of MicroRNA-21 in Macrophages in Response to Bacillus Calmette-Guerin Infection Involves Modulation of the TLR4/MyD88 Signaling Pathway. Cell Physiol Biochem 42:91-102 (2017). PubMed: 28494464