Anti-Endothelin A Receptor/ET-A抗体(ab117521)
Key features and details
- Rabbit polyclonal to Endothelin A Receptor/ET-A
- Suitable for: ICC, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Endothelin A Receptor/ET-A抗体
参阅全部 Endothelin A Receptor/ET-A 一抗 -
描述
兔多克隆抗体to Endothelin A Receptor/ET-A -
宿主
Rabbit -
经测试应用
适用于: ICC, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow, Dog, Pig -
免疫原
Synthetic peptide corresponding to Mouse Endothelin A Receptor/ET-A aa 400-500 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: Q61614 -
阳性对照
- Mouse dorsal root ganglia; Mouse heart tissue; Endothelin A Receptor/ET-A transfected cells; Mouse heart lysate
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常规说明
We have received mixed feedback about mouse cross reactivity, some lot works and some doesn't so we no longer guarantee this species.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99% Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab117521于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
1/5000.
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WB |
1/5000. Predicted molecular weight: 49 kDa.
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|
IHC-P |
1/2000.
|
说明 |
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ICC
1/5000. |
WB
1/5000. Predicted molecular weight: 49 kDa. |
IHC-P
1/2000. |
靶标
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功能
Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3. -
组织特异性
Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 281749 Cow
- Entrez Gene: 450187 Dog
- Entrez Gene: 1909 Human
- Entrez Gene: 13617 Mouse
- Entrez Gene: 397457 Pig
- GenBank: NM_001957 Human
- GenBank: NM_174308 Human
- Omim: 131243 Human
see all -
别名
- Ednra antibody
- EDNRA_HUMAN antibody
- Endothelin 1 receptor precursor antibody
see all
图片
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ab117521, at 1/5000 dilution, staining Endothelin A Receptor/ET-A in HEK293 transfected with Endothelin A receptor cells by Immunocytochemistry.
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ab117251, at 1/2000 dilution, staining Endothelin A Receptor/ET-A in the atrium of mouse heart by Immunohistochemistry.
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Anti-Endothelin A Receptor/ET-A antibody (ab117521) at 1/5000 dilution + Mouse heart lysate
Predicted band size: 49 kDa -
Immunohistochemistry analysis of mouse dorsal root ganglia labeling Endothelin A Receptor/ET-A with ab117521.
实验方案
数据表及文件
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Datasheet download
文献 (16)
ab117521 被引用在 16 文献中.
- Lee YJ et al. The EDN1/EDNRA/β‑arrestin axis promotes colorectal cancer progression by regulating STAT3 phosphorylation. Int J Oncol 62:N/A (2023). PubMed: 36453252
- Feng L et al. Hepatic Knockdown of Endothelin Type A Receptor (ETAR) Ameliorates Hepatic Insulin Resistance and Hyperglycemia Through Suppressing p66Shc-Mediated Mitochondrial Fragmentation in High-Fat Diet-Fed Mice. Diabetes Metab Syndr Obes 14:963-981 (2021). PubMed: 33688230
- Au M et al. Endothelin-1 induces chondrocyte senescence and cartilage damage via endothelin receptor type B in a post-traumatic osteoarthritis mouse model. Osteoarthritis Cartilage 28:1559-1571 (2020). PubMed: 32858189
- Adams KL et al. Endothelin-1 signaling maintains glial progenitor proliferation in the postnatal subventricular zone. Nat Commun 11:2138 (2020). PubMed: 32358570
- Kong H et al. Endothelin receptors promote schistosomiasis-induced hepatic fibrosis via splenic B cells. PLoS Pathog 16:e1008947 (2020). PubMed: 33075079