Anti-Alpha-synuclein (phospho Y125)抗体(ab10789)
Key features and details
- Rabbit polyclonal to Alpha-synuclein (phospho Y125)
- Suitable for: ELISA
- Isotype: IgG
概述
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产品名称
Anti-Alpha-synuclein (phospho Y125)抗体
参阅全部 Alpha-synuclein 一抗 -
描述
兔多克隆抗体to Alpha-synuclein (phospho Y125)该产品是 快线抗体,通过亲和纯化得到。对抗原肽有高灵敏度及特异性(ELISA测试)。 Read the terms of use »
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宿主
Rabbit -
种属反应性
预测可用于: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Corresponding Unmodified Peptide
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Isotype control
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Recombinant Protein
应用
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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说明 |
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ELISA
Use at an assay dependent concentration. |
靶标
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功能
May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. -
组织特异性
Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals. -
疾病相关
Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
Parkinson disease 1
Parkinson disease 4
Dementia Lewy body -
序列相似性
Belongs to the synuclein family. -
结构域
The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments. -
翻译后修饰
Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. -
细胞定位
Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons. - Information by UniProt
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数据库链接
- Entrez Gene: 6622 Human
- Entrez Gene: 20617 Mouse
- Entrez Gene: 29219 Rat
- Omim: 163890 Human
- SwissProt: P37840 Human
- SwissProt: O55042 Mouse
- SwissProt: P37377 Rat
- Unigene: 21374 Human
see all -
别名
- Alpha synuclein antibody
- Alpha-synuclein antibody
- Alpha-synuclein, isoform NACP140 antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab10789 被引用在 10 文献中.
- Magalhães P & Lashuel HA Opportunities and challenges of alpha-synuclein as a potential biomarker for Parkinson's disease and other synucleinopathies. NPJ Parkinsons Dis 8:93 (2022). PubMed: 35869066
- Akiyama T et al. Synthetic mRNA-based differentiation method enables early detection of Parkinson's phenotypes in neurons derived from Gaucher disease-induced pluripotent stem cells. Stem Cells Transl Med 10:572-581 (2021). PubMed: 33342090
- Fayyad M et al. Investigating the presence of doubly phosphorylated a-synuclein at tyrosine 125 and serine 129 in idiopathic Lewy body diseases. Brain Pathol 30:831-843 (2020). PubMed: 32324926
- Krejciova Z et al. Replication of multiple system atrophy prions in primary astrocyte cultures from transgenic mice expressing human a-synuclein. Acta Neuropathol Commun 7:81 (2019). PubMed: 31109379
- Vicente Miranda H et al. Posttranslational modifications of blood-derived alpha-synuclein as biochemical markers for Parkinson's disease. Sci Rep 7:13713 (2017). PubMed: 29057912
- Kiely AP et al. a-Synucleinopathy associated with G51D SNCA mutation: a link between Parkinson's disease and multiple system atrophy? Acta Neuropathol 125:753-69 (2013). ICC/IF ; Human . PubMed: 23404372
- Schiess MC et al. CSF from Parkinson disease patients differentially affects cultured microglia and astrocytes. BMC Neurosci 11:151 (2010). ICC/IF ; Human . PubMed: 21114836
- Dinh K et al. Fluorescence microscopy and 3D image reconstruction of cytokine initiated disruption of the Parkinson disease associated proteins alpha-synuclein, tau and ubiquitin in cultured glial cells. Cytokine 45:179-83 (2009). ICC/IF ; Human . PubMed: 19157893
- Chen L et al. Tyrosine and serine phosphorylation of alpha-synuclein have opposing effects on neurotoxicity and soluble oligomer formation. J Clin Invest : (2009). WB ; Drosophila melanogaster . PubMed: 19855133
- Bick RJ et al. Cytokines disrupt intracellular patterns of Parkinson's disease-associated proteins alpha-synuclein, tau and ubiquitin in cultured glial cells. Brain Res 1217:203-12 (2008). ICC/IF ; Human . PubMed: 18501880