Anti-MyD88抗体[4D6] (ab107585)
Key features and details
- Mouse monoclonal [4D6] to MyD88
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-MyD88抗体[4D6]
参阅全部 MyD88 一抗 -
描述
小鼠单克隆抗体[4D6] to MyD88 -
宿主
Mouse -
特异性
Espected to recognise Isoforms 1, 3, 4, 5, CRAa and CRAb. -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide, corresponding to amino acids 50-100 of Human MyD88 (NP_002459).
-
阳性对照
- human ovary, human prostate and Jurkat cell lysates and CF-7 cells. Flow Cyt (Intra): Jurkat cells
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
-
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
4D6 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab107585于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
Use 0.5µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB |
Use a concentration of 5 - 7 µg/ml. Predicted molecular weight: 33 kDa.
|
|
ICC/IF |
Use a concentration of 5 µg/ml.
|
说明 |
---|
Flow Cyt (Intra)
Use 0.5µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 5 - 7 µg/ml. Predicted molecular weight: 33 kDa. |
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
-
功能
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. -
组织特异性
Ubiquitous. -
疾病相关
Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age. -
序列相似性
Contains 1 death domain.
Contains 1 TIR domain. -
结构域
The intermediate domain (ID) is required for the phosphorylation and activation of IRAK. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 4615 Human
- Omim: 602170 Human
- SwissProt: Q99836 Human
- Unigene: 82116 Human
-
别名
- Mutant myeloid differentiation primary response 88 antibody
- MYD 88 antibody
- Myd88 antibody
see all
图片
-
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MyD88 knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: MOLT4 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab107585 observed at 37 kDa. Red - loading control, ab176560, observed at 52 kDa.
ab107585 was shown to recognize MyD88 when MyD88 knockout samples were used, along with additional cross-reactive bands. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. ab107585 and ab176560 (loading control to alpha tubulin) were diluted 5 µg/mL and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
Intracellular flow cytometric analysis of MyD88 antibody (ab107585) in Jurkat cells (0.5 ug). Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-MyD88 antibody. Secondary antibody- anti-mouse IgG PE conjugated.
-
Immunocytochemical analysis of MyD88 antibody (ab107585) in MCF-7 cells using an isotype control (top) and Myd88 Antibody (5 µg/ml; bottom).
-
All lanes : Anti-MyD88 antibody [4D6] (ab107585) at 5 µg/ml
Lane 1 : Human ovary cell lysate
Lane 2 : Human prostate cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Jurkat cell lysate with Immunizing peptide
Secondary
All lanes : Goat anti-mouse Ig HRP
Predicted band size: 33 kDa
实验方案
数据表及文件
-
Datasheet download
文献 (3)
ab107585 被引用在 3 文献中.
- Liu W et al. Heparin alleviates LPS-induced endothelial injury by regulating the TLR4/MyD88 signaling pathway. Exp Ther Med 22:1397 (2021). PubMed: 34650645
- Liu TY et al. Polygonatum sibiricum polysaccharides prevent LPS-induced acute lung injury by inhibiting inflammation via the TLR4/Myd88/NF-?B pathway. Exp Ther Med 20:3733-3739 (2020). PubMed: 32855724
- Sun Y et al. Exercise intervention alleviates nerve injury by the suppression of inflammatory mediator expression via the TLR4/NF-?B signaling pathway. Exp Ther Med 16:2922-2930 (2018). PubMed: 30233666