Anti-IREB2/IRP2抗体(ab106926)
Key features and details
- Goat polyclonal to IREB2/IRP2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-IREB2/IRP2抗体
参阅全部 IREB2/IRP2 一抗 -
描述
山羊多克隆抗体to IREB2/IRP2 -
宿主
Goat -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Pig, Orangutan -
免疫原
Synthetic peptide corresponding to Human IREB2/IRP2 aa 489-503 (internal sequence).
Sequence:C-SIHYEGSEYKLSHGS
Database link: NP_004127.1 -
阳性对照
- WB: Human Liver lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab106926 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106926于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 3 - 6 µg/ml.
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
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IHC-P
Use a concentration of 3 - 6 µg/ml. |
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA. -
序列相似性
Belongs to the aconitase/IPM isomerase family. -
翻译后修饰
Ubiquitinated and degraded by the proteasome in presence of high level of iron and oxygen. Ubiquitinated by a SCF complex containing FBXL5. Upon iron and oxygen depletion FBXL5 is degraded, preventing ubiquitination and allowing its RNA-binding activity. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 539404 Cow
- Entrez Gene: 3658 Human
- Entrez Gene: 64602 Mouse
- Entrez Gene: 100174315 Orangutan
- Entrez Gene: 100153993 Pig
- Entrez Gene: 64831 Rat
- Omim: 147582 Human
- SwissProt: P48200 Human
see all -
别名
- ACO3 antibody
- FLJ23381 antibody
- IRE BP2 antibody
see all
图片
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All lanes : Anti-IREB2/IRP2 antibody (ab106926) at 0.3 µg/ml
Lane 1 : Human Liver lysate in RIPA buffer
Lane 2 : Human Liver lysate in RIPA buffer with immunizing peptide
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 105 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour.
An additional band of unknown identity was consistently observed at 60kDa. This band was successfully blocked by incubation with the immunizing peptide.
数据表及文件
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Datasheet download
文献 (1)
ab106926 被引用在 1 文献中.
- Cloonan SM et al. Mitochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice. Nat Med 22:163-74 (2016). PubMed: 26752519