小鼠IGF1 ELISA试剂盒(ab100695)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 2.74 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Tissue Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠IGF1 ELISA试剂盒
参阅全部 IGF1 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma, Tissue Lysate -
检测类型
Sandwich (quantitative) -
灵敏度
< 4 pg/ml -
范围
2.74 pg/ml - 2000 pg/ml -
回收率
98 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 86.4 72% - 99% Serum 101.2 85% - 117% Plasma 106.6 85% - 114% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s IGF1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IGF1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse IGF1 coated on a 96- well plate. Standards and samples are pipetted into the wells and IGF1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IGF1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse IGF1 2 vials IGF1 Microplate (12 x 8 wells) 1 unit Recombinant Mouse IGF1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
疾病相关
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
序列相似性
Belongs to the insulin family. -
细胞定位
Secreted. - Information by UniProt
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别名
- IBP1
- IGF I
- IGF IA
see all -
数据库链接
- Entrez Gene: 16000 Mouse
- SwissProt: P05017 Mouse
- Unigene: 268521 Mouse
图片
数据表及文件
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SDS download
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Datasheet download
文献 (31)
ab100695 被引用在 31 文献中.
- Thyne KM & Salmon AB Metabolic benefits of methionine restriction in adult mice do not require functional methionine sulfoxide reductase A (MsrA). Sci Rep 12:5073 (2022). PubMed: 35332198
- Hill CM et al. FGF21 is required for protein restriction to extend lifespan and improve metabolic health in male mice. Nat Commun 13:1897 (2022). PubMed: 35393401
- Recinella L et al. Protective effects of growth hormone-releasing hormone analogs in DSS-induced colitis in mice. Sci Rep 11:2530 (2021). PubMed: 33510215
- Young K et al. Decline in IGF1 in the bone marrow microenvironment initiates hematopoietic stem cell aging. Cell Stem Cell 28:1473-1482.e7 (2021). PubMed: 33848471
- Minnis-Lyons SE et al. Notch-IGF1 signaling during liver regeneration drives biliary epithelial cell expansion and inhibits hepatocyte differentiation. Sci Signal 14:N/A (2021). PubMed: 34158399