概述

  • 产品名称Anti-AATF抗体
    参阅全部 AATF 一抗
  • 描述
    兔多克隆抗体to AATF
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 125 and 175 of human AATF (NP_036270.1).

  • 阳性对照
    • HeLa whole cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab72148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 62 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
  • 组织特异性Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
  • 序列相似性Belongs to the AATF family.
  • 翻译后修饰Hyperphosphorylated during the G1/S phase transition.
  • 细胞定位Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AATF antibody
    • AATF_HUMAN antibody
    • Apoptosis antagonizing transcription factor antibody
    • Apoptosis-antagonizing transcription factor antibody
    • CHE 1 antibody
    • CHE1 antibody
    • DED antibody
    • Protein AATF antibody
    • Rb binding protein Che 1 antibody
    • Rb-binding protein Che-1 antibody
    see all

Anti-AATF antibody 图像

  • All lanes : Anti-AATF antibody (ab72148) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 62 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • Detection of Human AATF by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72148 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

Anti-AATF antibody (ab72148)参考文献

ab72148 has not yet been referenced specifically in any publications.

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