概述

  • 产品名称Anti-AATF抗体
    参阅全部 AATF 一抗
  • 描述
    兔多克隆抗体to AATF
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A 12 amino acid synthetic peptide from near the carboxy terminus of human AATF.

  • 阳性对照
    • Human small intestine cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab39631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 62 kDa).
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.

靶标

  • 功能May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
  • 组织特异性Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
  • 序列相似性Belongs to the AATF family.
  • 翻译后修饰Hyperphosphorylated during the G1/S phase transition.
  • 细胞定位Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AATF antibody
    • AATF_HUMAN antibody
    • Apoptosis antagonizing transcription factor antibody
    • Apoptosis-antagonizing transcription factor antibody
    • CHE 1 antibody
    • CHE1 antibody
    • DED antibody
    • Protein AATF antibody
    • Rb binding protein Che 1 antibody
    • Rb-binding protein Che-1 antibody
    see all

Anti-AATF antibody 图像

  • Lane 1 : Anti-AATF antibody (ab39631) at 0.5 µg/ml
    Lane 2 : Anti-AATF antibody (ab39631) at 1 µg/ml
    Lane 3 : Anti-AATF antibody (ab39631) at 2 µg/ml

    Lane 1 : Human small intestine cell lysate
    Lane 2 : Human small intestine cell lysate
    Lane 3 : Human small intestine cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    anti-rabbit IgG HRP at 1/10000 dilution

    Predicted band size : 62 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    (Lanes read from left to right, 1, 2, 3.)
  • ab39631 at 10µg/ml staining AATF in small intestine by IHC

  • Immunofluorescence of AATF in Human Small Intestine cells using ab39631 at 20 ug/ml.

Anti-AATF antibody (ab39631)参考文献

This product has been referenced in:

See 1 Publication for this product

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