The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 45 kDa). 1 µg/mL of ab110992 was sufficient for detection of AARSD1 in 20µg of transiently transfected Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
功能Functions in trans to edit the amino acid moiety from incorrectly charged tRNA(Ala).
序列相似性Belongs to the class-II aminoacyl-tRNA synthetase family. Alax-L subfamily.