A431膜extract裂解液(ab30134)

概述

  • 产品名称A431膜extract裂解液
    参阅全部 A431 细胞裂解液
  • 常规说明Cell line: A431 (Human epithelial carcinoma).


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.

  • 经测试应用适用于: WBmore details

性能

  • 形式Liquid
  • 存放说明Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • 存储溶液Preservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • 裂解物说明Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
  • 研究领域
  • 背景A431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.

应用

Our Abpromise guarantee covers the use of ab30134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

A431 membrane extract lysate (ab30134)参考文献

ab30134 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"