Anti-A2BP1 / Fox1抗体(ab83574)


  • 产品名称Anti-A2BP1 / Fox1抗体
    参阅全部 A2BP1 / Fox1 一抗
  • 描述
    兔多克隆抗体to A2BP1 / Fox1
  • 经测试应用适用于: WB, ELISA, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide from within the N terminal amino acids 1-50: MNCEREQLRG NQEAAAAPDT MAQPYASAQF APPQNGIPAE YTAPHPHPAP, of Human A2BP1/ Fox1 (NP_001135805)

  • 阳性对照
    • MCF7 cell lysate



Our Abpromise guarantee covers the use of ab83574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.

IHC-FoFr Use at an assay dependent concentration.


  • 功能RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis.
  • 组织特异性Predominantly expressed in muscle and brain.
  • 序列相似性Contains 1 RRM (RNA recognition motif) domain.
  • 细胞定位Nucleus. Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2BP1 antibody
    • A2 BP1 antibody
    • A2BP 1 antibody
    • A2BP antibody
    • A2BP1 antibody
    • Ataxin 2 binding protein 1 antibody
    • Ataxin-2-binding protein 1 antibody
    • Ataxin2 binding protein 1 antibody
    • FOX 1 antibody
    • Fox-1 homolog A antibody
    • fox-1-like RNA-binding protein 1 antibody
    • Hexaribonucleotide binding protein 1 antibody
    • Hexaribonucleotide-binding protein 1 antibody
    • HRNBP 1 antibody
    • HRNBP1 antibody
    • RBFOX1 antibody
    • RFOX1_HUMAN antibody
    • RNA binding protein fox-1 homolog 1 antibody
    • RNA binding protein fox-1 homolog 1, C. elegans, homolog of, 1 antibody
    • RNA binding protein, fox-1 homolog (C. elegans) 1 antibody
    see all

Anti-A2BP1 / Fox1 antibody 图像

  • Anti-A2BP1 / Fox1 antibody (ab83574) at 1 µg/ml (in 5% skim milk/ PBS) + MCF7 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 43 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

Anti-A2BP1 / Fox1 antibody (ab83574)参考文献

ab83574 has not yet been referenced specifically in any publications.

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Thank you for contacting Abcam.

Under our Abpromise, which is valid for 6 months after delivery, we guarantee ab83574 to work in western blot, ELISA & IHC-FoFr on mouse and human tissue. We have gone through a number of different lots of t...

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Thank you for your enquiry regarding our two antibodies (ab83574 and ab94581). Generally, brain (CNS) cells express Fox1 at high levels particularly in the following region cerebral cortex, hippocampus and lateral ventricle. As the datasheets indic...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Coronal Brain)
Specification Coronal Brain
Fixative Paraformaldehyde
Permeabilization Yes - PBS + 0.1% Tween20
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

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提交于 Feb 24 2010