Anti-A1CF/ACF抗体(ab89050)
Key features and details
- Mouse polyclonal to A1CF/ACF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-A1CF/ACF抗体
参阅全部 A1CF/ACF 一抗 -
描述
小鼠多克隆抗体to A1CF/ACF -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human A1CF/ACF aa 1-600. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_620311.1 -
阳性对照
- Placenta or HeLa whole cell lysate (ab150035).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab89050于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. |
靶标
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功能
Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense-mediated decay. -
组织特异性
Widely expressed with highest levels in brain, liver, pancreas, colon and spleen. -
序列相似性
Contains 3 RRM (RNA recognition motif) domains. -
结构域
The RRM domains are necessary but not sufficient for binding to APOB mRNA. Additional residues in the pre-RRM and C-terminal regions are required for RNA-binding and for complementing APOBEC1 activity. -
细胞定位
Nucleus. Endoplasmic reticulum. Cytoplasm. Predominantly nuclear where it localizes to heterochromatin. Also cytoplasmic where it is found at the outer surface of the endoplasmic reticulum (By similarity). Shuttles between the nucleus and cytoplasm. May be transported into the nucleus by the nuclear import protein TNPO2/TRN2 or by APOBEC1. - Information by UniProt
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数据库链接
- Entrez Gene: 29974 Human
- SwissProt: Q9NQ94 Human
- Unigene: 282795 Human
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别名
- A1CF antibody
- A1CF_HUMAN antibody
- ACF antibody
see all
图片
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Anti-A1CF/ACF antibody (ab89050) at 1 µg/ml + Human placenta lysate at 50 µg
Predicted band size: 65 kDa -
Anti-A1CF/ACF antibody (ab89050) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 65 kDa -
All lanes : Anti-A1CF/ACF antibody (ab89050) at 1 µg/ml
Lane 1 : A1CF/ACF-transfected 293T cell lysate
Lane 2 : non transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 65 kDa
Observed band size: 65 kDa
数据表及文件
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Datasheet download
文献 (2)
ab89050 被引用在 2 文献中.
- Liu Q et al. High APOBEC1 Complementation Factor Expression Positively Modulates the Proliferation, Invasion, and Migration of Endometrial Cancer Cells Through Regulating P53/P21 Signaling Pathway. Cancer Biother Radiopharm N/A:N/A (2020). PubMed: 32818382
- Fossat N et al. C to U RNA editing mediated by APOBEC1 requires RNA-binding protein RBM47. EMBO Rep : (2014). PubMed: 24916387