存放说明 Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
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纯度 Immunogen affinity purified
纯化说明 Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
功能 Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.
组织特异性 Predominantly in urogenital tissues.
序列相似性 Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain. Contains 1 RBD (Ras-binding) domain.
Information by UniProt
A raf 1 antibody
A Raf proto oncogene serine/threonine protein kinase antibody
ARAF 1 antibody
Anti-A RAF (phospho Y301 + Y302) antibody 图像
Western blot - A RAF (phospho Y301 + Y302) antibody (ab59439)
All lanes : Anti-A RAF (phospho Y301 + Y302) antibody (ab59439) at 1/500 dilution Lane 1 : HeLa cell extract, treated with PMA (125ng/ml, 30 mins) Lane 2 : HeLa cell extract, treated with PMA (125ng/ml, 30 mins) with phosphopeptide Predicted band size : 68 kDa Observed band size : 68 kDa
Anti-A RAF (phospho Y301 + Y302) antibody (ab59439)参考文献
has not yet been referenced specifically in any publications.
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