Anti-A RAF (phospho Y301 + Y302)抗体(ab59439)

概述

  • 产品名称Anti-A RAF (phospho Y301 + Y302)抗体
    参阅全部 A RAF 一抗
  • 描述
    兔多克隆抗体to A RAF (phospho Y301 + Y302)
  • 特异性Detects endogenous levels of A RAF only when phosphorylated at tyrosine 301/tyrosine 302.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide (Human) from around the phosphorylation site of tyrosine 301/tyrosine 302 (GYYPWE) of A RAF.

  • 阳性对照
    • HeLa cell extract treated with PMA (125ng/ml, 30mins).
  • 常规说明Phosphorylation sites: Human: Tyr301/Tyr302; Mouse: Tyr299/Tyr300; Rat: Tyr299/Tyr300

性能

应用

Our Abpromise guarantee covers the use of ab59439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
ELISA 1/20000.

靶标

  • 功能Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.
  • 组织特异性Predominantly in urogenital tissues.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 RBD (Ras-binding) domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A raf 1 antibody
    • A Raf proto oncogene serine/threonine protein kinase antibody
    • ARAF 1 antibody
    • Araf antibody
    • ARaf proto oncogene serine/threonine protein kinase antibody
    • ARAF_HUMAN antibody
    • ARAF1 antibody
    • Oncogene Araf1 antibody
    • Oncogene PKS2 antibody
    • PKS 2 antibody
    • PKS antibody
    • PKS2 antibody
    • Proto oncogene Pks antibody
    • Proto-oncogene A-Raf antibody
    • Proto-oncogene A-Raf-1 antibody
    • Proto-oncogene Pks antibody
    • RAFA 1 antibody
    • RAFA1 antibody
    • Ras binding protein DA Raf antibody
    • Serine/threonine-protein kinase A-Raf antibody
    • v raf murine sarcoma 3611 viral oncogene homolog antibody
    • v raf murine sarcoma 3611 viral oncogene homolog 1 antibody
    • v raf oncogene homolog 1 (murine sarcoma 3611 virus) antibody
    see all

Anti-A RAF (phospho Y301 + Y302) antibody 图像

  • All lanes : Anti-A RAF (phospho Y301 + Y302) antibody (ab59439) at 1/500 dilution

    Lane 1 : HeLa cell extract, treated with PMA (125ng/ml, 30 mins)
    Lane 2 : HeLa cell extract, treated with PMA (125ng/ml, 30 mins) with phosphopeptide


    Predicted band size : 68 kDa
    Observed band size : 68 kDa

Anti-A RAF (phospho Y301 + Y302) antibody (ab59439)参考文献

ab59439 has not yet been referenced specifically in any publications.

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